Human OPA1 Antibody

Detection of Human OPA1 by Western Blot. Western Blot shows lysates of MCF‑7 human breast cancer cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for OPA1 at approximately 80 and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of OPA1 in Human Kidney. OPA1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the mitochondrial membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human OPA1 by Simple Western^TM. Simple Western shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for OPA1 at approximately 90 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB11631). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.