Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all OSMR beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OSM R beta
Glu28-Ser739
Accession # Q99650

Specificity

Detects human OSM R beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse OSM R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human OSMR beta Antibody

Detection of OSM R beta antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human OSM R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4389, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

OSM R beta Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

OSM R beta Specificity is Shown by Flow Cytometry in Knockout Cell Line.

OSM R beta knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human OSM R beta Affinity Purified Polyclonal Antibody (Catalog # AF4389, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by PE-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0107). No staining in the OSM R beta knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human OSMR beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Knockout Validated

0.25 µg/106 cells
Sample: OSM R beta is specifically detected in HeLa human carcinoma parental cell line but is not detectable in OSM R beta knockout HeLa cell line. 

Western Blot

0.1 µg/mL
Sample: Recombinant Human OSM R beta (Catalog # 4389-OR)

Reviewed Applications

Read 1 review rated 2 using AF4389 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: OSMR beta

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

References

  1. Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. 
  2. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 
  3. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. 
  4. Gearing, D.P. et al. (1992) Science 255:1434. 
  5. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. 
  6. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.  
  7. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. 
  8. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  9. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  10. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
  11. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
  12. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
  13. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
  14. Tanaka, M. et al. (2003) Blood 102:3154.
  15. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

Long Name

Oncostatin M Receptor beta

Alternate Names

OSM R beta, OSMR, OSMRB

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Gene Symbol

OSMR

UniProt

Q99650

Additional OSMR beta Products

Product Documents for Human OSMR beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human OSMR beta Antibody

For research use only

Customer Reviews for Human OSMR beta Antibody (1)

2 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
100%
1 Stars
0%

Have you used Human OSMR beta Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human OSMR beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates and Tissue Lysates
    Species: Human
    Verified Customer | Posted 05/06/2021
    Tried with various conditions on cells lysates without success by Western blot. However, when I tried an OSMR antibody from a different company on the same samples alongside, that worked no problem. The antibody was only tried for Western blot.
    Human OSMR beta Antibody AF4389

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies