Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all OSMR beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human OSM R beta
Glu28-Ser739
Accession # Q99650

Specificity

Detects human OSM R beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse OSM R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human OSMR beta Antibody

Detection of OSM R beta antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human OSM R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4389, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
OSM R beta Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

OSM R beta Specificity is Shown by Flow Cytometry in Knockout Cell Line.

OSM R beta knockout HeLa epithelial carcinoma cell line was stained with Mouse Anti-Human OSM R beta Affinity Purified Polyclonal Antibody (Catalog # AF4389, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by PE-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0107). No staining in the OSM R beta knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human OSMR beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Knockout Validated

0.25 µg/106 cells
Sample: OSM R beta is specifically detected in HeLa human carcinoma parental cell line but is not detectable in OSM R beta knockout HeLa cell line. 

Western Blot

0.1 µg/mL
Sample: Recombinant Human OSM R beta (Catalog # 4389-OR)

Reviewed Applications

Read 1 review rated 2 using AF4389 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OSMR beta

OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).

References

  1. Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. 
  2. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 
  3. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. 
  4. Gearing, D.P. et al. (1992) Science 255:1434. 
  5. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. 
  6. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.  
  7. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. 
  8. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  9. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  10. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273.
  11. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742.
  12. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135.
  13. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760.
  14. Tanaka, M. et al. (2003) Blood 102:3154.
  15. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

Long Name

Oncostatin M Receptor beta

Alternate Names

OSM R beta, OSMR, OSMRB

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Gene Symbol

OSMR

UniProt

Q99650

Additional OSMR beta Products

Product Documents for Human OSMR beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human OSMR beta Antibody

For research use only

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  • Human OSMR beta Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates and Tissue Lysates
    Species: Human
    Verified Customer | Posted 05/06/2021
    Tried with various conditions on cells lysates without success by Western blot. However, when I tried an OSMR antibody from a different company on the same samples alongside, that worked no problem. The antibody was only tried for Western blot.
    Human OSMR beta Antibody AF4389

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