Human Otolin-1 Antibody

Catalog # Availability Size / Price Qty
MAB8045
MAB8045-SP
Detection of Human Otolin-1 by Western Blot.
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Human Otolin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Otolin-1 in ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 839418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Otolin-1
Lys24-Pro477
Accession # A6NHN0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Otolin-1 antibody by Western Blot. View Larger

Detection of Human Otolin-1 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells undifferentiated or differentiated to early otic lineage. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Otolin-1 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Otolin-1 antibody in differentiated BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Otolin-1 in differentiated BG01V Human Embryonic Stem Cells. Otolin-1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to early otic lineage using Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otolin-1

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear. It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function. It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including Cerebellin-1 and Otoconin-90. Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus. It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions.  Human Otolin shares 72% aa sequence identity with mouse and rat Otolin.

Entrez Gene IDs
131149 (Human); 229389 (Mouse); 295117 (Rat)
Alternate Names
C1q and tumor necrosis factor related protein 15; C1qTNF15; LOC131149; OTOL1; otolin 1; Otolin-1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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