Human Otolin-1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Otolin-1 in ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 839418
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Otolin-1
    Lys24-Pro477
    Accession # A6NHN0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Otolin-1 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells undifferentiated or differentiated to early otic lineage. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Otolin-1 Monoclonal Antibody (Catalog # MAB8045) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Otolin-1 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Otolin-1 in differentiated BG01V Human Embryonic Stem Cells. Otolin-1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to early otic lineage using Mouse Anti-Human
Otolin-1 Monoclonal Antibody (Catalog # MAB8045) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Otolin-1

Otolin (OTOL1), also known as C1qTNF15, is an approximately 65 kDa protein found in the otoconial membrane lining the cochlea and vestibular labyrinth of the inner ear. It is secreted by supporting cells of the sensory epithelium. The otoconial membrane contains particles known as otoconia which are composed of glycoproteins and proteoglycans coated with calcium carbonate crystals. Otolin is one of the protein components of otoconia particles, and it is important for otoconia formation as well as for auditory and vestibular function. It associates into multimers and disulfide-linked oligomers and also associates with other otoconial proteins including Cerebellin-1 and Otoconin-90. Otolin contains three collagen-like regions followed by a C1q-like domain at the C-terminus. It is extensively glycosylated and has multiple hydroxylated proline residues in the collagenous regions.  Human Otolin shares 72% aa sequence identity with mouse and rat Otolin.

  • Entrez Gene IDs:
    131149 (Human); 229389 (Mouse); 295117 (Rat)
  • Alternate Names:
    C1q and tumor necrosis factor related protein 15; C1qTNF15; LOC131149; OTOL1; otolin 1; Otolin-1
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