p18INK4c (18 kDa Inhibitor of CDK4-c; also p18-INK6 and cyclin-dependent kinase 6 inhibitor) is an 18-19 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is expressed in skeletal muscle, macrophages, T cells and B cells where it serves as a negative regulator of cell proliferation. It does so by specifically associating with either CDK4 or, principally, CDK6, thereby blocking cyclin binding and the activity that leads to a G1-to-S cell cycle transition. In the case of B cells, this activity is necessary for the development of plasma cells. Human p18INK4c is 168 amino acids (aa) in length. It contains four "L" shaped ankyrin repeats (aa 4-132) that interact with cyclin. The C-terminus is described as containing a fifth ankyrin repeat, one that may act to reverse cell cycle repression. Full-length human p18INK4c shares 92% aa sequence identity with mouse p18INK4c.
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala2-Gln168
Accession # P42773
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human p18INK4c/CDKN2C Antibody
Detection of Human p18INK4c/CDKN2C by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p18INK4c/CDKN2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7746) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p18INK4c/CDKN2C at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human p18INK4c/CDKN2C Antibody
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: p18INK4c/CDKN2C
Long Name
Alternate Names
Gene Symbol
UniProt
Additional p18INK4c/CDKN2C Products
Product Documents for Human p18INK4c/CDKN2C Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human p18INK4c/CDKN2C Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars