Human p18INK4c/CDKN2C Antibody

Catalog # Availability Size / Price Qty
AF7746
AF7746-SP
Detection of Human p18INK4c/CDKN2C by Western Blot.
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Human p18INK4c/CDKN2C Antibody Summary

Species Reactivity
Human
Specificity
Detects human p18INK4c/CDKN2C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human p19INK4d is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p18INK4c/CDKN2C
Ala2-Gln168
Accession # P42773
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human p18INK4c/CDKN2C by Western Blot. View Larger

Detection of Human p18INK4c/CDKN2C by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p18INK4c/CDKN2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7746) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p18INK4c/CDKN2C at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p18INK4c/CDKN2C

p18INK4c (18 kDa Inhibitor of CDK4-c; also p18-INK6 and cyclin-dependent kinase 6 inhibitor) is an 18-19 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is expressed in skeletal muscle, macrophages, T cells and B cells where it serves as a negative regulator of cell proliferation. It does so by specifically associating with either CDK4 or, principally, CDK6, thereby blocking cyclin binding and the activity that leads to a G1-to-S cell cycle transition. In the case of B cells, this activity is necessary for the development of plasma cells. Human p18INK4c is 168 amino acids (aa) in length. It contains four "L" shaped ankyrin repeats (aa 4-132) that interact with cyclin. The C-terminus is described as containing a fifth ankyrin repeat, one that may act to reverse cell cycle repression. Full-length human p18INK4c shares 92% aa sequence identity with mouse p18INK4c.

Long Name
p18 Cyclin Dependent Kinase 4 Inhibitor 2C
Entrez Gene IDs
1031 (Human); 12580 (Mouse); 54238 (Rat)
Alternate Names
CDK6 inhibitor p18; CDKN2C; CDKN6; cyclin-dependent inhibitor; cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4); INK4Ccyclin-dependent kinase 6 inhibitor p18; p18; p18INK4c; p18-INK4C; p18-INK6

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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