|Detection of Human p18INK4c/CDKN2C by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p18INK4c/CDKN2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7746) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p18INK4c/CDKN2C at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
p18INK4c (18 kDa Inhibitor of CDK4-c; also p18-INK6 and cyclin-dependent kinase 6 inhibitor) is an 18-19 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is expressed in skeletal muscle, macrophages, T cells and B cells where it serves as a negative regulator of cell proliferation. It does so by specifically associating with either CDK4 or, principally, CDK6, thereby blocking cyclin binding and the activity that leads to a G1-to-S cell cycle transition. In the case of B cells, this activity is necessary for the development of plasma cells. Human p18INK4c is 168 amino acids (aa) in length. It contains four "L" shaped ankyrin repeats (aa 4-132) that interact with cyclin. The C-terminus is described as containing a fifth ankyrin repeat, one that may act to reverse cell cycle repression. Full-length human p18INK4c shares 92% aa sequence identity with mouse p18INK4c.
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