Human p53R2 Antibody

Detection of Human p53R2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of p53R2 by Western Blot Gemcitabine resistance is driven by RRM1 overexpression. A) Quantification of relative RRM1 mRNA abundance following shRNA-mediated knock-down in BxPC3, BxGR-80C and BxGR-360C cells. Three independent RNA samples analyzed per cell type. b) Relative gemcitabine IC50 values following RRM1 knock-down, normalized to the control for each subclone. c) Correlation between relative RRM1 mRNA abundance and absolute gemcitabine (GEM) IC50 for all RRM1 knock-down cells and controls. d) Western blotting of dose-dependent induction of an RRM1 band with the apparent mass of 110–120 kDa (RRM1*) and RRM2 in gemcitabine treated cells. a-b) Statistical significance tested using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of p53R2 by Western Blot Gemcitabine resistance is driven by RRM1 overexpression. A) Quantification of relative RRM1 mRNA abundance following shRNA-mediated knock-down in BxPC3, BxGR-80C and BxGR-360C cells. Three independent RNA samples analyzed per cell type. b) Relative gemcitabine IC50 values following RRM1 knock-down, normalized to the control for each subclone. c) Correlation between relative RRM1 mRNA abundance and absolute gemcitabine (GEM) IC50 for all RRM1 knock-down cells and controls. d) Western blotting of dose-dependent induction of an RRM1 band with the apparent mass of 110–120 kDa (RRM1*) and RRM2 in gemcitabine treated cells. a-b) Statistical significance tested using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of p53R2 by Western Blot Confirmation of protein-level overexpression and copy number alteration in gemcitabine resistant sub-clones. A-b) Western blotting analysis of RRM1, RRM2, RRM2B and deoxycytidine kinase (dCK) in BxPC3 and derived subclones BxGR-80C, BxGR-120C and BxGR-360C. c) RRM1 TaqMan Copy Number Analysis of BxPC3 and derived BxGR-80C and BxGR-360C subclones. HUVEC cells used as diploid reference control. Three independent DNA samples analyzed per cell type d) Estimation of relative RRM1 mRNA abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. e) Estimation of RRM1 protein abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. f) Correlation between RRM1 copy-number, RRM1 mRNA and protein abundance for BxPC3, BxGR-80C and BxGR-360C cells. Solid lines are linear regressions, r2 is the coefficient of determination. g-h) Western blotting of E-cadherin, STIM1 and RhoG in BxPC3 and gemcitabine resistant subclones. c-e) Statistical significance performed using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean and n indicates independent samples. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of p53R2 by Western Blot Confirmation of protein-level overexpression and copy number alteration in gemcitabine resistant sub-clones. A-b) Western blotting analysis of RRM1, RRM2, RRM2B and deoxycytidine kinase (dCK) in BxPC3 and derived subclones BxGR-80C, BxGR-120C and BxGR-360C. c) RRM1 TaqMan Copy Number Analysis of BxPC3 and derived BxGR-80C and BxGR-360C subclones. HUVEC cells used as diploid reference control. Three independent DNA samples analyzed per cell type d) Estimation of relative RRM1 mRNA abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. e) Estimation of RRM1 protein abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. f) Correlation between RRM1 copy-number, RRM1 mRNA and protein abundance for BxPC3, BxGR-80C and BxGR-360C cells. Solid lines are linear regressions, r2 is the coefficient of determination. g-h) Western blotting of E-cadherin, STIM1 and RhoG in BxPC3 and gemcitabine resistant subclones. c-e) Statistical significance performed using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean and n indicates independent samples. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.