Human p53R2 Antibody

Detection of p53R2 by Western Blot

Confirmation of protein-level overexpression and copy number alteration in gemcitabine resistant sub-clones. A-b) Western blotting analysis of RRM1, RRM2, RRM2B and deoxycytidine kinase (dCK) in BxPC3 and derived subclones BxGR-80C, BxGR-120C and BxGR-360C. c) RRM1 TaqMan Copy Number Analysis of BxPC3 and derived BxGR-80C and BxGR-360C subclones. HUVEC cells used as diploid reference control. Three independent DNA samples analyzed per cell type d) Estimation of relative RRM1 mRNA abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. e) Estimation of RRM1 protein abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. f) Correlation between RRM1 copy-number, RRM1 mRNA and protein abundance for BxPC3, BxGR-80C and BxGR-360C cells. Solid lines are linear regressions, r2 is the coefficient of determination. g-h) Western blotting of E-cadherin, STIM1 and RhoG in BxPC3 and gemcitabine resistant subclones. c-e) Statistical significance performed using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean and n indicates independent samples. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of p53R2 by Western Blot

Confirmation of protein-level overexpression and copy number alteration in gemcitabine resistant sub-clones. A-b) Western blotting analysis of RRM1, RRM2, RRM2B and deoxycytidine kinase (dCK) in BxPC3 and derived subclones BxGR-80C, BxGR-120C and BxGR-360C. c) RRM1 TaqMan Copy Number Analysis of BxPC3 and derived BxGR-80C and BxGR-360C subclones. HUVEC cells used as diploid reference control. Three independent DNA samples analyzed per cell type d) Estimation of relative RRM1 mRNA abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. e) Estimation of RRM1 protein abundance in BxGR-80C and BxGR-360C relative to BxPC3 cells. f) Correlation between RRM1 copy-number, RRM1 mRNA and protein abundance for BxPC3, BxGR-80C and BxGR-360C cells. Solid lines are linear regressions, r2 is the coefficient of determination. g-h) Western blotting of E-cadherin, STIM1 and RhoG in BxPC3 and gemcitabine resistant subclones. c-e) Statistical significance performed using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean and n indicates independent samples. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.