Detection of Human p53R2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The p53-inducible ribonucleotide reductase (p53R2) shares similarity with the R2 subunit of ribonucleotide reductase. Both function to provide dNTPs for DNA synthesis. p53R2 is induced in response to genotoxic stress, whereas the R2 functions under homeostatic conditions. When the expression of p53R2 is decreased, cells exhibit decreased ribonucleotide reductase activity, DNA repair activity, and cellular survival in response to genotoxins.
Entrez Gene IDs:
50484 (Human); 382985 (Mouse)
EC 126.96.36.199; MGC102856; MGC42116; MTDPS8A; MTDPS8B; p53-inducible ribonucleotide reductase small subunit 2 homolog; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; P53R2DKFZp686M05248; PEOA5; ribonucleoside-diphosphate reductase subunit M2 B; ribonucleotide reductase M2 B (TP53 inducible); RRM2B; TP53-inducible ribonucleotide reductase M2 B
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