Human Park7/DJ-1 Antibody

Catalog # Availability Size / Price Qty
MAB3995
MAB3995-SP
Detection of Human Park7/DJ‑1 by Western Blot.
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Reviews (3)

Human Park7/DJ-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Park7 in Western blots.
Source
Monoclonal Rat IgG2A Clone # 421015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Park7
Met1-Asp189
Accession # Q99497
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
25 µg/mL
LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line
Knockout Validated
Park7/DJ‑1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Park7/DJ‑1 knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Park7/DJ‑1 antibody by Western Blot. View Larger

Detection of Human Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, LNCaP human prostate cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Park7/DJ‑1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Park7/DJ‑1 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ‑1 at approximately 26 kDa (as indicated) using 25 µg/mL of Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human Park7/DJ-1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Park7/DJ-1

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions, including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.

Long Name
Parkinson Disease 7
Entrez Gene IDs
11315 (Human); 57320 (Mouse); 117287 (Rat)
Alternate Names
DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1

Product Datasheets

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Reviews for Human Park7/DJ-1 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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3 Star
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Human Park7/DJ-1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Park7/DJ-1 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Park7/DJ-1 Antibody
By Anonymous on 11/09/2018
Application: Microarray Sample Tested: EDTA Plasma Species: Human