Human Park7/DJ-1 Antibody

Detection of Human Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, LNCaP human prostate cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Park7/DJ‑1 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ‑1 at approximately 26 kDa (as indicated) using 25 µg/mL of Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Park7/DJ-1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Park7/DJ‑1 Specificity Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7/DJ‑1 knockout HEK293T cell line (KO). Nitrocellulose membrane was probed with Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995) followed by HRP-conjugated secondary antibody. A specific band was detected for Park7/DJ‑1 at approximately 20 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. Primary antibody dilution used: 0.5µg. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Park7/DJ‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HEK293T human embryonic kidney parental cell line WT and Park7/DJ‑1 HEK293T KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rat Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB3995) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).