Human PAUF/ZG16B Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PAUF/ZG16B in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 817310
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PAUF/ZG16B
    Gly53-Arg208
    Accession # Q96DA0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PAUF/ZG16B by Western Blot. Western blot shows lysates of human placenta tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PAUF/ZG16B at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PAUF/ZG16B in Human Pancreas Cancer Tissue. PAUF/ZG16B was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAUF/ZG16B
PAUF (pancreatic adenocarcinoma upregulated factor), gene name ZG16B (zymogen granule protein 16B), is a 156 amino acid, approximately 20-25 kDa secreted protein that is a member of the jacalin-binding lectin family. PAUF expression is thought to be restricted to primates, but the related ZG16P, which shares approximately 25% amino acid sequence identity, is more widely expressed. Human PAUF is overexpressed in pancreatic adenocarcinoma, binds Toll-like receptors TLR2 and TLR4, and inhibits CXCR4-dependent, TLR2-mediated NF-kB activation. It facilitates tumor growth, adhesiveness, and production of pro-tumorigenic cytokines.
  • Long Name:
    Pancreatic Adenocarcinoma Upregulated Factor
  • Entrez Gene IDs:
    124220 (Human)
  • Alternate Names:
    DKFZp762P2216; EC 3.1.2; EC 3.4.19.12; EECP; HRPE773; hTrabid; JCLN2; LOC124220; PAUF; PRO1567; TRABIDubiquitin thioesterase ZRANB1; TRAF-binding protein domain; ZG16B; Zinc finger Ran-binding domain-containing protein 1; zinc finger, RAN-binding domain containing 1
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