Key Product Details

Species Reactivity

Human

Applications

Western Blot, Blockade of Receptor-ligand Interaction, Neutralization, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2340D
View all PD-L1/B7-H1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human PD-L1/B7-H1 protein
Phe19-Thr239
Accession # Q9NZQ7

Specificity

Detects human PD-L1/B7-H1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human PD-L1/B7-H1 Antibody

Detection of human PD-L1/B7-H1 antibody by Western Blot.

Detection of human PD-L1/B7-H1 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PD-L1/B7-H1 antibody in HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP antibody by Flow Cytometry.

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human PD-L1/B7-H1 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). View our protocol for Staining Membrane-associated Proteins.

PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody.

In a functional flow cytometry test, Recombinant Human PD-1 His-tagged protein (8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC1050A).

PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody.

In a functional flow cytometry test, Recombinant Human PD-1 Fc Chimera Alexa Fluor® 647 protein (AFR1086, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 25 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PD-L1/B7-H1 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional flow cytometry test, 2.5 μg/mL of Rabbit anti-human PD-L1/B7-H1 Antibody(Catalog # MAB1562) will block the binding of Recombinant Human PD-1 His-tagged protein (Catalog # 8986-PD) to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP

Western Blot

2 µg/mL
Sample: Human Lung Tissue

Neutralization

In a functional ELISA binding assay, 0.2-1.2 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Human PD-L1/B7-H1 (Catalog # 156-B7) to immobilized Recombinant Human PD-1 Fc chimera (Catalog # 1086-PD) coated at 100 ng/mL (100 µL/well). At 2-3 µg/mL, this antibody will block >90% of the binding.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on
inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Dong, H. et al. (1999) Nat. Med. 5:1365.
  3. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  4. Tamura, H. et al. (2001) Blood 97:1809.
  5. Chen, L. et al. (2007) J. Immunol. 178:6634.
  6. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  7. Tsushima, F. et al. (2007) Blood 110:180.
  8. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  9. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  10. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  11. Dong, H. et al. (2002) Nat. Med. 8:793.
  12. Azuma, T. et al. (2008) Blood 111:3635.
  13. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  14. Park, J.-J. et al. (2010) Blood 116:1291.
  15. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  16. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9NZQ7

Additional PD-L1/B7-H1 Products

Product Documents for Human PD-L1/B7-H1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PD-L1/B7-H1 Antibody

For research use only

Citations for Human PD-L1/B7-H1 Antibody

Customer Reviews for Human PD-L1/B7-H1 Antibody

There are currently no reviews for this product. Be the first to review Human PD-L1/B7-H1 Antibody and earn rewards!

Have you used Human PD-L1/B7-H1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies