Human PEDFR/PNPLA2 Antibody

Catalog # Availability Size / Price Qty
PEDF R/PNPLA2 in Human Heart.
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Product Details
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Human PEDFR/PNPLA2 Antibody Summary

Species Reactivity
Detects human PEDF R/PNPLA2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse PNPLA2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PEDF R/PNPLA2
Accession # Q96AD5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PEDF R/PNPLA2 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

PEDF R/PNPLA2 in Human Heart. PEDF R/PNPLA2 was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human PEDF R/ PNPLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5387) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEDFR/PNPLA2

PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55-60 kDa member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle, and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Human PEDF R is described as being a 504 amino acid (aa) type II transmembrane (TM) protein. Its TM segment is stated to span aa 9-29. It also contains a palatin domain (aa 10-179) with a conserved GxSxG enzymatic motif. There is one potential isoform variant that shows a deletion of aa 2-62. Over aa 162-332, human PEDF R shares 85% aa identity with mouse PEDF R.

Long Name
Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2
Entrez Gene IDs
57104 (Human); 66853 (Mouse); 361676 (Rat)
Alternate Names
Adipose triglyceride lipase; ATGL; ATGL1110001C14Rik; Calcium-independent phospholipase A2; Desnutrin; EC; FP17548; IPLA2-zeta; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2-like; PEDF R; PEDFR; PEDF-R; Pigment epithelium-derived factor; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2; TTS-2.2; TTS2DKFZp667M109

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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