Human Pentraxin 3/TSG-14 Antibody

(1 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Pentraxin 3/TSG‑14 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Pentraxin 3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Pentraxin 3/TSG‑14
    Glu18-Ser381
    Accession # P26022
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Pentraxin 3/TSG‑14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pentraxin 3/TSG‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pentraxin 3/TSG‑14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1‑3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).

Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23‑28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly up‑regulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG‑14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

  • References:
    1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
    2. Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
    3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
    4. Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
    5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
    6. Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
    7. Introna, M. et al. (1996) Blood 87:1862.
    8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
    9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
    10. Rovere, P. et al. (2000) Blood 96:4300.
    11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
    12. Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.
  • Long Name:
    Tumor Necrosis Factor Stimulated Gene 14
  • Entrez Gene IDs:
    5806 (Human); 19288 (Mouse)
  • Alternate Names:
    alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Interleukin-1 in combination with oncostatin M up-regulates multiple genes in chondrocytes: implications for cartilage destruction and repair.
    Authors: Barksby HE, Hui W, Wappler I, Peters HH, Milner JM, Richards CD, Cawston TE, Rowan AD
    Arthritis Rheum., 2006;54(2):540-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 applications: ELISA, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Human Anonymous 12/01/2017
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Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesHuman
Immunocytochemistry/Immunofluorescence Human Pentraxin 3/TSG-14 Antibody AF1826
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Very Good
 ICC/IF Mouse Anonymous 10/12/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human Pentraxin 3/TSG-14 Antibody AF1826
Immunocytochemistry/Immunofluorescence: Human Pentraxin 3/TSG-14 Antibody [AF1826]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedFibrotic Lung
SpeciesMouse

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