Peptide YY in Human Pancreas.
Peptide YY was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB9456) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peptide YY
Peptide YY (PYY), a secretory peptide, is expressed by L
cells (enteroendocrine cells) in the small and large intestine, Islet of
Langerhans cells, testis, and in some central and peripheral neurons. Because
PYY is released from L cells after feeding, it is thought to produce a satiety
signal. While the regulation of appetite by PYY and GLP-1 have been studied
extensively, recent evidence supports a role for PYY in Islet of Langerhans beta
cell survival. PYY secretion is also modulated by gut microbiota, and levels of
colonic PYY have reduced in rats with DSS-induced colitis, and in humans with
inflammatory bowel disease (IBD), while circulating PYY increased in IBD.
Entrez Gene IDs:
5697 (Human); 217212 (Mouse); 287730 (E. faecalis)
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