Detection of Human Peroxiredoxin 3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Peroxiredoxin 3 Monoclonal Antibody (Catalog # MAB7504) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Peroxiredoxin 3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Peroxiredoxin 3 in HeLa Human Cell Line. Peroxiredoxin 3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Peroxiredoxin 3 Monoclonal Antibody (Catalog # MAB7504) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 3
Peroxiredoxin 3 (Prx-3/III; also AOP-1, MER5 and thioredoxin-dependent peroxidase reductase) is a ubiquitous, 22-28 kDa mitochondrial antioxidant enzyme that belongs to the 2-Cys class of the TSA/ahpC family of peroxiredoxins. Prx-3 is known to act as either a homodimer, or a decamer, and scavenge reactive oxygen species generated by oxidative stress. The mouse Prx-3 precursor molecule is 257 amino acids (aa) in length. It contains a cleavable N-terminal 63 aa mitochondrial targeting sequence, plus a 194 aa mature enzyme that shows a thioredoxin domain between aa 64-222. There are two catalytic cysteines, one at Cys109 and another at Cys230 of the precursor. Prx-3 undergoes a phosphorylation at Thr147 that reduces its activity. One potential splice form shows a deletion of Arg149, Lys150 and Arg185. Full-length mouse Prx-3 is 86% and 95% aa identical to human and rat Prx-3, respectively.
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