Human Phospho-Chk2 (T68) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1626-SP
MAB1626-100
Detection  of Human Chk2 by Western Blot.
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Human Phospho-Chk2 (T68) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chk2 when phosphorylated at T68 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1238H
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant human Phospho-Chk2 (T68)
Accession # O96017
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
2-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human Chk2 by Western Blot. View Larger

Detection of Human Chk2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 μM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Chk2 (T68) Monoclonal Antibody (Catalog # MAB1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Chk2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-Chk2  (T68) in HeLa Human Cell Line. View Larger

Phospho-Chk2 (T68) in HeLa Human Cell Line. Chk2phosphorylated at T68 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line unstimulated (lower panel) or stimulated with 1 μM Camptothecin (upper panel) using Rabbit Anti-Human Phospho-Chk2 (T68) Monoclonal Antibody (Catalog # MAB1626) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei (upper panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Chk2

Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.

Long Name
Checkpoint Kinase 2
Entrez Gene IDs
11200 (Human); 50883 (Mouse); 114212 (Rat)
Alternate Names
CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

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