Human Phospho-EGFR (Y1086) Antibody

Detection of Phospho-EGFR (Y1086) by Western Blot

Western blot analysis of p-CREB, CREB, p-EGFR, EGFR, p-ERK1/2, ERK1, ERK2, EP4, and MRP4 in OVCAR-5 cells. Cells were treated with the indicated agents for 24 h, then light-activated (0.1 J/cm2, 10 mW/cm2) or maintained in dark conditions. After 24 h, cells were agonized with EGF (50 ng/mL) and PGE2 (1 µM) for 10 min, then whole extracts were collected and analyzed using Western blot. (A) Representative Western blot images and (B–K) relative densitometric bar graphs of phosphorylated and total proteins were shown. Results are normalized to the vehicle control group. Statistical analysis was performed using a one-way ANOVA and post hoc Tukey’s test. Percentages below each band represent the average change in intensity relative to the vehicle control across all experiments. For pERK1 and pERK2 bands, the first number corresponds to pERK1, and the second number corresponds to pERK2. Error bars represent the standard error of the mean. * p ≤ 0.05; ** p ≤ 0.01; ns: nonsignificant. Original western blot images (Supplementary Figure S5). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34771424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Phospho-EGFR (Y1086) by Western Blot

Western blot analysis of p-CREB, CREB, p-EGFR, EGFR, p-ERK1/2, ERK1, ERK2, EP4, and MRP4 in OVCAR-5 cells. Cells were treated with the indicated agents for 24 h, then light-activated (0.1 J/cm2, 10 mW/cm2) or maintained in dark conditions. After 24 h, cells were agonized with EGF (50 ng/mL) and PGE2 (1 µM) for 10 min, then whole extracts were collected and analyzed using Western blot. (A) Representative Western blot images and (B–K) relative densitometric bar graphs of phosphorylated and total proteins were shown. Results are normalized to the vehicle control group. Statistical analysis was performed using a one-way ANOVA and post hoc Tukey’s test. Percentages below each band represent the average change in intensity relative to the vehicle control across all experiments. For pERK1 and pERK2 bands, the first number corresponds to pERK1, and the second number corresponds to pERK2. Error bars represent the standard error of the mean. * p ≤ 0.05; ns: nonsignificant. Original western blot images (Supplementary Figure S4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34771424), licensed under a CC-BY license. Not internally tested by R&D Systems.