Detection of Human and Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 5 minutes and 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB1825) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-ERK1 (T202/Y204) and Phospho-ERK2 (T185/Y187) at approximately 43 and 41 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ERK1 and ERK2 (also
known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively.
They are part of the Ras-Raf-ERK signal transduction cascade often found
downstream of growth factor receptor activation. ERK1 and ERK2 were initially
isolated and cloned as kinases activated in response to insulin and NGF. They
are expressed in most, if not all, mammalian tissues. Dual threonine and
tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1
and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share
100% amino acid sequence identity.
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