Human Phospho-FGF R1-4 (Y653/Y654) Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FGF R when dually phosphorylated at Y653/Y654 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human FGF R1 Y653/Y654 sites
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-
FGF R1‑4 (Y653/Y654) by Western Blot.
Western blot shows lysates of KATO‑III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom­binant Human FGF acidic (Catalog #
232‑FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1‑4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1‑4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Phospho-FGF R1-4 (Y653/Y654) in A431 Human Cell Line. FGF R1‑4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1‑4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R1-4
The fibroblast growth factor receptors (FGF R), including FGF R1, R2, R3, and R4, are involved in development processes, angiogenesis, wound healing, and tumorigenesis. FGF binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Tyr653 and Tyr654 in FGF R1 and the corresponding tyrosine residues in FGF R2, R3 and R4 are important for the activation of intrinsic kinase activity.
  • Long Name:
    Fibroblast Growth Factor Receptors 1-4
  • Alternate Names:
    FGF R1-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress
    Authors: D Nawrocka, K Kornicka, J Szydlarska, K Marycz
    Oxid Med Cell Longev, 2017;2017(0):3027109.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival.
    Authors: Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B
    Cancer Res., 2008;68(7):2340-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
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