Human Phospho-FGFR1-4 (Y653/Y654) Antibody

Catalog # Availability Size / Price Qty
AF3285
AF3285-SP
Detection of Human Phospho- FGF R1‑4 (Y653/Y654) by Western Blot.
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Product Details
Citations (5)
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Human Phospho-FGFR1-4 (Y653/Y654) Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF R when dually phosphorylated at Y653/Y654 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human FGF R1 Y653/Y654 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho- FGF R1-4 (Y653/Y654) antibody by Western Blot. View Larger

Detection of Human Phospho- FGF R1‑4 (Y653/Y654) by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recom-binant Human FGF acidic (Catalog # 232-FA) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FGF R1-4 (Y653/Y654) at approximately 120 to 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-FGF R1-4 (Y653/Y654) antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-FGF R1-4 (Y653/Y654) in A431 Human Cell Line. FGF R1-4 phosphorylated at Y653/Y654 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-FGF R1-4 (Y653/Y654) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3285) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR1-4

The fibroblast growth factor receptors (FGF R), including FGF R1, R2, R3, and R4, are involved in development processes, angiogenesis, wound healing, and tumorigenesis. FGF binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Tyr653 and Tyr654 in FGF R1 and the corresponding tyrosine residues in FGF R2, R3 and R4 are important for the activation of intrinsic kinase activity.

Long Name
Fibroblast Growth Factor Receptors 1-4
Alternate Names
FGFR1-4

Product Datasheets

Citations for Human Phospho-FGFR1-4 (Y653/Y654) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Transferrin receptor 1 promotes the fibroblast growth factor receptor-mediated oncogenic potential of diffused-type gastric cancer
    Authors: T Shirakihar, H Yamaguchi, T Kondo, M Yashiro, R Sakai
    Oncogene, 2022;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth
    Authors: L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu
    Cell Death Dis, 2018;9(3):262.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. FGFR2 amplification is predictive of sensitivity to regorafenib in gastric and colorectal cancers in vitro
    Authors: Y Cha, HP Kim, Y Lim, SW Han, SH Song, TY Kim
    Mol Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress
    Authors: D Nawrocka, K Kornicka, J Szydlarska, K Marycz
    Oxid Med Cell Longev, 2017;2017(0):3027109.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival.
    Authors: Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B
    Cancer Res., 2008;68(7):2340-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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