Human Phospho-IKK alpha (S176/S180) Antibody

Catalog # Availability Size / Price Qty
Detection of Human Phospho-IKK alpha  (S176/S180) by Western Blot.
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Product Details
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Human Phospho-IKK alpha (S176/S180) Antibody Summary

Species Reactivity
Detects human IKK alpha when phosphorylated at S176/S180.
Monoclonal Rat IgG2A Clone # 829177
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing the human IKK alpha S176/S180
Accession # O15111
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-IKKa (S176/S180) antibody by Western Blot. View Larger

Detection of Human Phospho-IKK alpha (S176/S180) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-IKKa (S176/S180) Mono-clonal Antibody (Catalog # MAB3768) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-IKKa (S176/S180) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK alpha

I kappa B kinase alpha (IKK alpha ) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.

Long Name
IkB Kinase alpha
Entrez Gene IDs
1147 (Human); 12675 (Mouse); 309361 (Rat)
Alternate Names
CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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