Human Phospho-MSK1 (S212) Antibody

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Detection of Human Phospho-MSK1 (S212) by Western Blot.
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Product Details
Citations (1)
Supplemental Products
Reviews (3)

Human Phospho-MSK1 (S212) Antibody Summary

Species Reactivity
Detects human MSK1 when phosphorylated at S212.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human MSK1 S212 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-MSK1 (S212) by Western Blot. View Larger

Detection of Human Phospho-MSK1 (S212) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM H2O2for 1 hour or 300 mM sorbitol for 30 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1 (S212) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MSK1 in Human Kidney Cancer Tissue. View Larger

MSK1 in Human Kidney Cancer Tissue. MSK1 phosphorylated at S212 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name
Mitogen- and Stress-activated Protein Kinase 1
Entrez Gene IDs
9252 (Human); 73086 (Mouse)
Alternate Names
EC 2.7.11; MGC1911,90 kDa ribosomal protein S6 kinase 5; MSK1; MSK1EC; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kD, polypeptide 5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RPS6KA5; RSKL; RSK-like protein kinase; S6K-alpha-5

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Citation for Human Phospho-MSK1 (S212) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells.
    Authors: Tamguney T, Zhang C, Fiedler D, Shokat K, Stokoe D
    Exp. Cell Res., 2008;314(11):2299-312.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Reviews for Human Phospho-MSK1 (S212) Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human Phospho-MSK1 (S212) Antibody
By Anonymous on 04/06/2022
Application: IHC Sample Tested: Melanoma tissue Species: Human

Tissue was cryopreserved

Human Phospho-MSK1 (S212) Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Phospho-MSK1 (S212) Antibody
By Anonymous on 10/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human