Human Phospho-PDGF R beta DuoSet IC ELISA
Human Phospho-PDGF R beta DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Product Datasheets
Preparation and Storage
Background: PDGF R beta
PDGF R beta is a transmembrane receptor tyrosine kinase (RTK) that primarily binds to the PDGF-BB isoform of the growth factor. Ligand binding induces receptor homodimerization or heterodimerization with PDGF R alpha followed by autophosphorylation and signal transduction. PDGF receptor signaling is attenuated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. The expression of the alpha and beta receptors is independently regulated in various cell types. A soluble splice variant of PDGF R beta binds PDGF with high affinity and functions as a potent PDGF antagonist.
Citations for Human Phospho-PDGF R beta DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis.
Authors: Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B
J Pharmacol Exp Ther, 2014;349(2):209-20.
Species: Human
Sample Types: Cell Lysates
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Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130Cas.
Authors: Pellet-Many C, Frankel P, Evans IM, Herzog B, Junemann-Ramirez M, Zachary IC
Biochem. J., 2011;435(3):609-18.
Species: Human
Sample Types: Cell Lysates
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Neuropilin-1 signaling through p130Cas tyrosine phosphorylation is essential for growth factor-dependent migration of glioma and endothelial cells.
Authors: Evans IM, Yamaji M, Britton G, Pellet-Many C, Lockie C, Zachary IC, Frankel P
Mol. Cell. Biol., 2011;31(6):1174-85.
Species: Human
Sample Types: Cell Lysates
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The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain.
Authors: Martino MM, Hubbell JA
FASEB J., 2010;24(12):4711-21.
Species: Human
Sample Types: Cell Lysates
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Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007;177(3):489-500.
Species: Human
Sample Types: Cell Lysates
FAQs
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Which phosphorylated sites are recognized by this assay?
This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
DuoSet Ancillary Reagent Kits
Supplemental ELISA Products
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