Human PI 3-Kinase p110 gamma Antibody

Name: Human PI 3-Kinase p110 gamma Antibody
Brand: R&D Systems
SKU: MAB2999
Price: 419 USD
Availability: InStock

R&D Systems | Catalog # MAB2999

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse, Lobster

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 304103

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Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p110 gamma
Lys298-Leu467
Accession # P48736

Specificity

Detects endogenous human PI 3-Kinase p110 gamma in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human PI 3-Kinase p110 gamma Antibody

Detection of Human PI 3-Kinase p110 gamma by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 gamma Monoclonal Antibody (Catalog # MAB2999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PI 3-Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PI 3-Kinase p110 gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p110 gamma

Class I phosphoinositide 3-kinases (PI 3-Kinases) are dual-specific lipid and protein kinases involved in numerous intracellular signaling pathways. Class IA PI 3‑Kinases (p110 alpha, beta and delta ) are typically activated through receptor tyrosine kinase signaling, whereas p110 gamma, the single member of class IB, is activated by seven‑transmembrane G‑protein‑coupled receptors (GPCRs). While p110 alpha and beta are ubiquitiously expressed, expression of p110 gamma is mainly restricted to cells of the hematopoietic system.