Human PKR Antibody MAB1980: R&D Systems

Human PKR Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PKR in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # HL71/10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Protein kinase fraction from interferon-treated Daudi human Burkitt's lymphoma cell line. Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunoprecipitation
    Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PKR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018).
A specific band was detected for PKR at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
PKR in HeLa Human Cell Line. PKR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with rhIFN-alpha (Catalog # 11110-1) using Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human PKR by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKR at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKR
The interferon (IFN)-induced PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic inititation factor 2 (elF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappa B.
  • References:
    1. Bonnet, M.C. et al. (2000) Mol. Cell Biol. 20:4532.
  • Long Name:
    Protein Kinase R
  • Entrez Gene IDs:
    5610 (Human); 19106 (Mouse); 54287 (Rat)
  • Alternate Names:
    double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P68 Kinase; PKR; PKRp68 kinase; PPP1R83; PRKR; PRKRMGC126524; Protein Kinase R; Protein kinase RNA-activated
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Altered activation of protein kinase PKR and enhanced apoptosis in dystonia cells carrying a mutation in PKR activator protein PACT.
    Authors: Vaughn L, Bragg D, Sharma N, Camargos S, Cardoso F, Patel R
    J Biol Chem, 2015;290(37):22543-57.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  2. Essential role of PACT-mediated PKR activation in tunicamycin-induced apoptosis.
    Authors: Singh M, Fowlkes V, Handy I, Patel CV, Patel RC
    J. Mol. Biol., 2009;385(2):457-68.
    Species: Human
    Sample Type: Whole Cells
    Application: IP
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