Human PKR Antibody

Catalog # Availability Size / Price Qty
MAB1980
MAB1980-SP
Detection of Human PKR by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Human PKR Antibody Summary

Species Reactivity
Human
Specificity
Detects human PKR in Western blots.
Source
Monoclonal Mouse IgG1 Clone # HL71/10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Protein kinase fraction from interferon-treated Daudi human Burkitt's lymphoma cell line. Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunoprecipitation
Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PKR antibody by Western Blot. View Larger

Detection of Human PKR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKR at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PKR antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

PKR in HeLa Human Cell Line. PKR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with rhIFN-alpha (Catalog # 11110-1) using Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human PKR antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human PKR by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKR at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKR

The interferon (IFN)-induced PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic inititation factor 2 (elF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappa B.

References
  1. Bonnet, M.C. et al. (2000) Mol. Cell Biol. 20:4532.
Long Name
Protein Kinase R
Entrez Gene IDs
5610 (Human); 19106 (Mouse); 54287 (Rat)
Alternate Names
double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P68 Kinase; PKR; PKRp68 kinase; PPP1R83; PRKR; PRKRMGC126524; Protein Kinase R; Protein kinase RNA-activated

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Citations for Human PKR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Regulation of PKR activation and apoptosis during oxidative stress by TRBP phosphorylation
    Authors: B Ukhueduan, E Chukwurah, RC Patel
    The international journal of biochemistry & cell biology, 2021;137(0):106030.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Stress-induced TRBP phosphorylation enhances its interaction with PKR to regulate cellular survival
    Authors: E Chukwurah, RC Patel
    Sci Rep, 2018;8(1):1020.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The Noncoding RNA nc886 Regulates PKR Signaling and Cytokine Production in Human Cells
    Authors: E Golec, L Lind, M Qayyum, AM Blom, BC King
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Altered activation of protein kinase PKR and enhanced apoptosis in dystonia cells carrying a mutation in PKR activator protein PACT.
    Authors: Vaughn L, Bragg D, Sharma N, Camargos S, Cardoso F, Patel R
    J Biol Chem, 2015;290(37):22543-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Essential role of PACT-mediated PKR activation in tunicamycin-induced apoptosis.
    Authors: Singh M, Fowlkes V, Handy I, Patel CV, Patel RC
    J. Mol. Biol., 2009;385(2):457-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PKR Antibody

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Human PKR Antibody
By Anonymous on 10/20/2021
Application: ICC/IF Sample Tested: Mitotic cells Species: Human

Human PKR Antibody
By Anonymous on 07/25/2021
Application: WB Sample Tested: HeLa cells Species: Human

Human PKR Antibody
By Anonymous on 11/18/2019
Application: WB Sample Tested: HeLa cells Species: Human