Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HL71/10
View all PKR Primary Antibodies »

Product Specifications

Immunogen

Protein kinase fraction from interferon-treated Daudi human Burkitt's lymphoma cell line. Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.

Specificity

Detects human PKR in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PKR Antibody

Detection of Human PKR antibody by Western Blot.

Detection of Human PKR by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKR at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PKR antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

PKR in HeLa Human Cell Line.

PKR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with rhIFN-alpha (Catalog # 11110-1) using Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human PKR antibody by Simple WesternTM.

Detection of Human PKR by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKR at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human PKR Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with universal IFN-alpha (Catalog # 11200-1) or recombinant human IFN-alpha (Catalog # 11100-1). Besse, S. et al. (1998) Exp. Cell Res. 239:379.

Immunoprecipitation

Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.

Simple Western

10 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line

Reviewed Applications

Read 3 reviews rated 5 using MAB1980 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKR

The interferon (IFN)-induced PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic inititation factor 2 (elF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappa B.

References

  1. Bonnet, M.C. et al. (2000) Mol. Cell Biol. 20:4532.

Long Name

Protein Kinase R

Alternate Names

EIF2AK2, P68 Kinase, PPP1R83, PRKR, Protein Kinase R

Entrez Gene IDs

5610 (Human); 19106 (Mouse); 54287 (Rat)

Gene Symbol

EIF2AK2

Additional PKR Products

Product Documents for Human PKR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PKR Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human PKR Antibody

Customer Reviews for Human PKR Antibody (3)

5 out of 5
3 Customer Ratings
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human PKR Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mitotic cells
    Species: Human
    Verified Customer | Posted 10/20/2021
    Human PKR Antibody MAB1980
  • Human PKR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 07/25/2021
    Human PKR Antibody MAB1980
  • Human PKR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 11/18/2019
    Human PKR Antibody MAB1980

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Protocols

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FAQs

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