Human PLA2G7/PAF-AH/Lp-PLA2 Antibody Summary
Accession # AAH38452
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PLA2G7/PAF-AH/Lp-PLA2 by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLA2G7/PAF-AH/Lp-PLA2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human PLA2G7/PAF‑AH/Lp‑PLA2 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 1:10 dilution. A specific band was detected for PLA2G7/PAF-AH/Lp-PLA2 at approximately 33 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Secretory phopholipase A2 is an enzyme that hydrolyses the Sn-2 ester bond of phospholipids, generating free fatty acids and lysophospholipids (1‑3). Most secretory PLA2s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. Also known as Lp-PLA2, PLA2G-VII is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%‑20% to HDL, and the remainder to VLDL (4‑6). It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s, PLA2G‑VII has poor specificity toward Sn-2 long chain fatty acids, unless heavily oxidized, and undergoes the catalysis of its substrates in the aqueous phase rather than at the interfacial surface of lipids. Thus, it has high specificity for water-soluble phospholipids in plasma including oxidatively‑modified phospholipids and platelet-activating factor (PAF). Because of the latter activity, it is also known as PAF acetylhydrolase (PAF-AH). Lack of human PLA2G‑VII is related to a higher risk for stroke and heart disease.
- Webb, N. R. (2005) Cur. Opin. Lipid. 16:341.
- Triggiani, M. et al. (2005) J. Allergy Clin. Immunol. 116:1000.
- Murakami, M. and Kudo, I. (2004) Biol. Pharm. Bull. 27:1158.
- Caslake, M. J. and Packard C. J. (2005) Nat. Clin. Prac. Cardiovasc. Med. 2:529.
- Karabina, S.-A., and Ninio, E. (2006) Biochim. Biophys. Acta, 1761:1351.
- Karasawa, K. (2006) Biochim. Biophys. Acta, 1761:1359.
Citation for Human PLA2G7/PAF-AH/Lp-PLA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Differential expression of Lp-PLA2 in obesity and type 2 diabetes and the influence of lipids
Authors: L Jackisch, W Kumsaiyai, JD Moore, N Al-Daghri, I Kyrou, TM Barber, H Randeva, S Kumar, G Tripathi, PG McTernan
Sample Types: Tissue Homogenates
Applications: Western Blot
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