Detects human PLA2G7/PAF-AH/Lp-PLA2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PLA2G-2A, recombinant mouse PLA2G-2A, and rhPLA2G-1B is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human PLA2G7/PAF-AH/Lp-PLA2 Met33-Asn441 Accession # AAH38452
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human PLA2G7/PAF-AH/Lp-PLA2 by Western Blot.
Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLA2G7/PAF-AH/Lp-PLA2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human PLA2G7/PAF‑AH/Lp‑PLA2 by Simple WesternTM.
Simple Western lane view shows human plasma, loaded at 1:10 dilution. A specific band was detected for PLA2G7/PAF‑AH/Lp‑PLA2 at approximately 33 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLA2G7/PAF‑AH/Lp‑PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Secretory phopholipase A2 is an enzyme that hydrolyses the Sn-2 ester bond of phospholipids, generating free fatty acids and lysophospholipids (1‑3). Most secretory PLA2s are stored in cytoplasmic granules and are released in the extracellular environment on appropriate cell activation. Thus, they are present at higher concentration in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic disease, such as acute pancreatitis, rheumatoid arthritis, bronchial asthma, and allergic rhinitis. Also known as Lp-PLA2, PLA2G-VII is a plasma enzyme bound to lipoproteins: 80% bound to LDL, 15%‑20% to HDL, and the remainder to VLDL (4‑6). It is produced in major by mature macrophages and activated platelets. In contrast to other classical sPLA2s, PLA2G‑VII has poor specificity toward Sn-2 long chain fatty acids, unless heavily oxidized, and undergoes the catalysis of its substrates in the aqueous phase rather than at the interfacial surface of lipids. Thus, it has high specificity for water-soluble phospholipids in plasma including oxidatively‑modified phospholipids and platelet-activating factor (PAF). Because of the latter activity, it is also known as PAF acetylhydrolase (PAF-AH). Lack of human PLA2G‑VII is related to a higher risk for stroke and heart disease.
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Karasawa, K. (2006) Biochim. Biophys. Acta, 1761:1359.
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