Human Plasminogen/Plasmin Antibody

Catalog # Availability Size / Price Qty
MAB2596
MAB2596-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human Plasminogen/Plasmin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plasminogen and Plasmin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 270412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived Plasminogen
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human Plasminogen (Catalog # 1939-SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasminogen

Plasminogen (PLG) is the precursor of Plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, Plasminogen is found in plasma and many extracellular fluids. Activated by u- or t-Plasminogen Activator, the single‑chain Plasminogen (aa 20-810) is converted to Plasmin, which consists of disulfide bond‑linked heavy chain A (aa 20-580) and light chain B (aa 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named Angiostatin, an angiogenesis inhibitor (3, 4).

References
  1. Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
  2. Forsgren, M. et al. (1987) FEBS Lett. 213:254.
  3. O’Reilly, M.S. et al. (1994) Cell 79:315.
  4. Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Entrez Gene IDs
5340 (Human); 18815 (Mouse); 85253 (Rat)
Alternate Names
DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; Plasminogen; Plg

Product Datasheets

Citations for Human Plasminogen/Plasmin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Exposure of HL-60 human leukaemic cells to 4-hydroxynonenal promotes the formation of adduct(s) with alpha-enolase devoid of plasminogen binding activity.
    Authors: Gentile F, Pizzimenti S, Arcaro A, Pettazzoni P, Minelli R, D'Angelo D, Mamone G, Ferranti P, Toaldo C, Cetrangolo G, Formisano S, Dianzani MU, Uchida K, Dianzani C, Barrera G
    Biochem. J., 2009;422(2):285-94.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  2. Immunogenic and plasminogen-binding surface-associated alpha-enolase of Trichomonas vaginalis.
    Authors: Mundodi V, Kucknoor AS, Alderete JF
    Infect. Immun., 2007;76(2):523-31.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Fibrin derived from patients with chronic thromboembolic pulmonary hypertension is resistant to lysis.
    Authors: Morris TA, Marsh JJ, Chiles PG, Auger WR, Fedullo PF, Woods VL
    Am. J. Respir. Crit. Care Med., 2006;173(11):1270-5.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Plasminogen/Plasmin Antibody

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Human Plasminogen/Plasmin Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma