Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 846224
View all PLD2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLD2
Met1-His117
Accession # O14939

Specificity

Detects huma PLD2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PLD2 Antibody

Detection of Human PLD2 antibody by Western Blot.

Detection of Human PLD2 by Western Blot.

Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PLD2 Monoclonal Antibody (Catalog # MAB10123) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PLD2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLD2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLD2

Phospholipase D2 (PLD2) is an enzyme encoded by the PLD2 gene. Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. The Phospholipase D (PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different PLD2 isoforms -PLD2A, PLD2B and PLD2C- have been described, ranging from 38 to 105 KDa.

Long Name

Phospholipase D2

Alternate Names

Choline Phosphatase 2, HPLD2, PLD1C

Entrez Gene IDs

5338 (Human); 18806 (Mouse); 25097 (Rat)

Gene Symbol

PLD2

UniProt

O14939

Additional PLD2 Products

Product Documents for Human PLD2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLD2 Antibody

For research use only

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