Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 559819
View all Plexin B1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157

Specificity

Detects human Plexin B1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Plexin B1 Antibody

Plexin B1 antibody in Human Prostate by Immunohistochemistry (IHC-P).

Plexin B1 in Human Prostate.

Plexin B1 was detected in immersion fixed paraffin-embedded sections of human prostate using Human Plexin B1 Monoclonal Antibody (Catalog # MAB37492) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Plexin B1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors.  Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region.  The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats.  The ECD is cleaved into two subunits, a 200 kDa a-chain (aa 20-1305) and a 100 kDa TM b-chain.  The subunits are nondisulfide-linked and generate a high-affinity receptor.  Plexin B1 is a receptor for Semaphorin 4D/CD100.  It forms a receptor complex with Neuropilins, MET, and EGF R2.  Multiple splice variants are known. One shows a deletion of aa 689-871.  A second shows a 53 aa substitution for aa 677-2135.  And a third shows a 53 aa substitution for the 53 aa between aa 677-729.  Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.  

  

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

O43157

Additional Plexin B1 Products

Product Documents for Human Plexin B1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Plexin B1 Antibody

For research use only

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Protocols

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