Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Plexin B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Plexin B1
Leu35-Val150
Accession # O43157

Specificity

Detects human Plexin B1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Plexin B3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Plexin B1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

0.1 µg/mL
Sample: Recombinant Human Plexin B1

Reviewed Applications

Read 1 review rated 4 using AF3749 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin B1

Plexin B1 is a type I transmembrane protein that belongs to the Plexin family of semaphorin receptors. It is derived from a single peptide that undergoes proteolytic processing in its extracellular domain to form the non-covalently-linked heterodimer of the extracellular alpha subunit and the transmembrane beta subunit. Human Plexin B1 extracellular alpha subunit shares homology with semaphorins, HGF receptor and MSP receptor and contains a sema domain and three IPT/TIG domains. It functions as a receptor for semaphorin 4D/CD100. Multiple splice isoforms of Plexin B1 have been reported. Within the region used as immunogen, human Plexin B1 shares 90% and 97% amino acid sequence homology with mouse and canine Plexin B1, respectively.

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

O43157

Additional Plexin B1 Products

Product Documents for Human Plexin B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Plexin B1 Antibody

For research use only

Citations for Human Plexin B1 Antibody

Customer Reviews for Human Plexin B1 Antibody (1)

4 out of 5
1 Customer Rating
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1 Stars
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  • Human Plexin B1 Antibody
    Name: Yong Huang
    Application: Western Blot
    Sample Tested: E11.5 mouse whole embryo lysate
    Species: Mouse
    Verified Customer | Posted 11/23/2016
    Human Plexin B1 Antibody AF3749

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Protocols

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FAQs

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