CD90/Thy1 Antibody

R&D Systems | Catalog # AF2067

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine

Cited:

Human, Porcine, Canine

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Flow Cytomery

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD90/Thy1
Gln20-Ser141
Accession # P04216

Specificity

Detects human CD90/Thy1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD90 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for CD90/Thy1 Antibody

Detection of Human CD90/Thy1 antibody by Western Blot.

Detection of Human CD90/Thy1 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD90/Thy1 at approximately 22-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD90/Thy1 antibody in BG01V Human Embyonic Stem Cells by Immunocytochemistry (ICC).

CD90/Thy1 in BG01V Human Embyonic Stem Cells.

CD90/Thy1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
CD90/Thy1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

CD90/Thy1 in Human Prostate Cancer Tissue.

CD90/Thy1 was detected in formalin fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CD90/Thy1 antibody in Canine Mesenchymal Stem Cells by Immunocytochemistry (ICC).

CD90/Thy1 in Canine Mesenchymal Stem Cells.

CD90/Thy1 was detected in immersion fixed canine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
CD90/Thy1 antibody in Porcine Mesenchymal Stem Cells by Immunocytochemistry (ICC).

CD90/Thy1 in Porcine Mesenchymal Stem Cells.

CD90/Thy1 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of CD90/Thy1 antibody in Human Mesenchymal Stem Cells antibody by Flow Cytometry.

Detection of CD90/Thy1 in Human Mesenchymal Stem Cells by Flow Cytometry.

Human mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Detection of CD90/Thy1 antibody in Porcine Mesenchymal Stem Cells antibody by Flow Cytometry.

Detection of CD90/Thy1 in Porcine Mesenchymal Stem Cells by Flow Cytometry.

Porcine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Detection of CD90/Thy1 antibody in Canine Mesenchymal Stem Cells antibody by Flow Cytometry.

Detection of CD90/Thy1 in Canine Mesenchymal Stem Cells by Flow Cytometry.

Canine mesenchymal stem cells were stained with Sheep Anti-Human/Porcine/Canine CD90/Thy1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2067, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by PE-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Detection of bshq0d8ep9smtgobgp8t.jpg by Immunohistochemistry

Detection of bshq0d8ep9smtgobgp8t.jpg by Immunohistochemistry

Expression of PDPN and CD90 in head and neck paraganglioma (HNPGN) tissue on immunohistochemistry. Immunohistochemistry images of HNPGN tissue. CD90 is in brown and podoplanin (PDPN) is in pink, nuclei are blue. (A) PDPN and CD90 expression in HNPGN sample from patient PGN010. Left panel is at 20x magnification while the right panel show 40x magnification of the tissue area in the white box inset. (B) PDPN and CD90 staining as above in HNPGN tissue from patient PGN09. Arrows point to CD90 stained cells and arrowheads to the PDPN stained cells (C) Control staining (left panel) and positive staining (right panel) with CD90 (brown) and PDPN (pink) is displayed in tissue from synovium in rheumatoid arthritis (RA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39619326), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD90/Thy1 by Flow Cytometry

Detection of CD90/Thy1 by Flow Cytometry

Characterization of 3,2′-DHF-treated WJ-MSCs and EVs. (A) The proliferation rate of WJ-MSC treated with various concentrations of 3,2′-DHF. WJ-MSCs have the best proliferation rate with 1–2 μM 3,2′-DHF. Statistical analysis was determined using one-way ANOVA with Tukey’s multiple comparisons test: ** p < 0.05, **** p < 0.0001. (B) Protein expression of MEK/ERK signaling proteins in WJ-MSCs with and without 3,2′-DHF treatment. The bar graph shows the protein expression of AKT and MEK in 3,2′-DHF-treated and untreated WJ-MSCs. (C) Flow cytometry analysis to confirm the characteristics of WJ-MSCs with or without 3,2′-DHF. The expression of the positive markers CD73, CD90, and CD105, and the negative markers CD34 and CD45, was assessed. (D) The relative EV production amounts of Cont-EV and Fla-EV were measured using nanoparticle tracking analysis (NTA). Statistical analysis was determined using two-way ANOVA: **** p < 0.0001. (E) Transmission electron microscopy (TEM) images of Cont-EV and Fla-EV. Scale bar: 100 nm. The size of the EVs was determined with NTA. (F) Characterization of EV-positive markers CD9 and CD63, and negative markers calnexin and GM130, in Cont-EV and Fla-EV using Western blotting. (G) Representative plot of EV-surface markers CD63 and CD81 in Cont-EV and Fla-EV via flow cytometry analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37108128), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD90/Thy1 by Flow Cytometry

Detection of CD90/Thy1 by Flow Cytometry

Characterization of Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) after treatment with wild-type basic fibroblast growth factor (WT-bFGF) or thermostable bFGF (TS-bFGF). (A) Phase-contrast microscopy images of WJ-MSCs and WJ-MSCs treated with WT-bFGF (WT-WJ-MSCs) and WJ-MSCs treated with TS-bFGF (TS-WJ-MSCs). Scale bar: 200 μm. (B) Flow cytometry analysis of positive marker (CD73, CD90, and CD105) and negative marker (CD34 and CD45) expression in WT-WJ-MSCs and TS-WJ-MSCs. (C) OCT4, SOX2, NANOG, and KLF4 gene expression levels in WT-WJ-MSCs and TS-WJ-MSCs over 3 days. The mRNA expression levels were determined using quantitative RT-PCR and normalized to the control group. (D) Fibroblast colony-forming unit (CFU-F) assays were performed to assess CFU-F formation in WT-WJ-MSCs and TS-WJ-MSCs, and the results are presented in photographs and graphs. The data are presented as the mean ± standard deviation (n = 3). ns: not significant. * p < 0.05, ** p < 0.01, and **** p < 0.0001 compared to the control group and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 in comparison to the WT-3D EV group. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/22/16460), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD90/Thy1 by Immunocytochemistry/ Immunofluorescence

Detection of CD90/Thy1 by Immunocytochemistry/ Immunofluorescence

ILC2s are increased in human HPS lungs.(A) ILC2 staining in human lung tissues. Anti-GATA3, -CD90/Thy1, and a cocktail of 4 antibodies against CD3, CD56, CD20, and CD79 alpha were employed to stain human lung tissues in normal individuals, idiopathic pulmonary fibrosis (IPF) patients, and HPS patients. ILC2s are identified as GATA3+CD90/Thy1+, and antibody cocktail–negative cells (purple cells). The corresponding phase-contrast image shows lung tissue architecture in normal individuals, and pathologic changes in IPF and HPS lungs. (B) Counting of ILC2s (normalized per area) under ×40 magnification using an immunofluorescence microscope. Values are mean ± SEM with a minimum of 4 samples in each group. Comparisons between groups were conducted by 2-way ANOVA with Bonferroni’s post hoc test. ***P ≤ 0.001. Scale bars: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39405112), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for CD90/Thy1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µL/106 cells
Sample: Human mesenchymal stem cells, porcine mesenchymal stem cells, and canine mesenchymal stem cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells, canine mesenchymal stem cells and porcine mesenchymal stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human prostate cancer tissue

Western Blot

1 µg/mL
Sample: Human brain (cortex) tissue, human brain (hippocampus) tissue, and human brain (hypothalamus) tissue

Reviewed Applications

Read 1 review rated 5 using AF2067 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD90/Thy1

CD90 (also Thy1/Thymus cell antigen 1) is a 25-35 kDa glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed on neurons, ovarian follicular cells, endothelial cells, fibroblasts and circulating CD34+ stem cells, and appears to act as an adhesion molecule. CD90 is known to bind to integrins beta 2, beta 5 and beta 3, the latter often accompanied by additional binding to syndecan-4. In the postnatal nervous system, this inhibits neurite outgrowth while stabilizing newly formed axonal networks. In the vascular system, CD90 mediates the extravasation of leukocytes. And in lung, a CD90: alpha v beta 5 interaction inhibits the extracellular activation of latent TGF-beta. Mature human CD90 is a 111 amino acid (aa) GPI-linked protein (aa 20-130). It contains one V-type Ig-like domain (aa 20-126) with a heparin-binding motif (aa 56-60), and a GPI-anchor amidated cysteine at position 130. The integrin binding site consists of an Arg35LeuAsp37 tripeptide. CD90 apparently forms 50‑60 kDa homodimers and 150 kDa homomultimers. There is one potential isoform variant that contains a 12 aa substitution for aa 1-13. Over aa 20-141, human CD90 shares 64% aa identity with mouse CD90 and 84% or 83% with horse and dog respectively.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

P04216

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody

Certificate of Analysis

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Product Specific Notices for CD90/Thy1 Antibody

For research use only

Citations for CD90/Thy1 Antibody

Customer Reviews for CD90/Thy1 Antibody (1)

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: HMSC cells
    Species: Human
    Verified Customer | Posted 01/07/2016
    HMSC cells stained CD90 antibody
    CD90/Thy1 Antibody AF2067

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