Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 528322
View all PP2C gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355

Specificity

Detects human PP2C gamma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PP2C gamma Antibody

Detection of Human PP2C?/PPM1G antibody by Western Blot.

Detection of Human PP2C gamma /PPM1G by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human PP2C gamma/PPM1G Monoclonal Antibody (Catalog # MAB5595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C gamma/PPM1G at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human PP2C gamma Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue

Western Blot

0.25 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB5595 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C gamma

Protein phosphatase 2C isoform gamma (PP2C gamma; also PPM1G) is a widely expressed, 75 kDa member of the PP2C family of Ser/Thr protein phosphatases. Human PP2C gamma is 546 amino acids (aa) in length. The apparent mass is much larger than the predicted mass of 59 kDa because of the presence of an acidic region (aa 259‑326) rich in glutamate and aspartate residues, causing the protein to move anomalously on SDS-PAGE. The protein contains numerous sites for phosphorylation by casein kinase II, particularly within the acidic domain.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Entrez Gene IDs

5496 (Human); 14208 (Mouse); 259229 (Rat)

Gene Symbol

PPM1G

UniProt

O15355 (Human)

Additional PP2C gamma Products

Product Documents for Human PP2C gamma Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PP2C gamma Antibody

For research use only

Customer Reviews for Human PP2C gamma Antibody (1)

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  • Human PP2C gamma Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 05/20/2022
    Human PP2C gamma Antibody MAB5595

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Protocols

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