Human Pregnancy Zone Protein/PZP Antibody Summary
Accession # P20742
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This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB82801).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pregnancy Zone Protein/PZP DuoSet ELISA Kit (Catalog # DY8280-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Pregnancy Zone Protein/PZP by Western Blot. Western blot shows lysate of human pregnancy sera. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Pregnancy Zone Protein/PZP ELISA Standard Curve. Recombinant Human Pregnancy Zone Protein/PZP protein was serially diluted 2-fold and captured by Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB82801) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pregnancy Zone Protein/PZP
Pregnancy Zone Protein (PZP), also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6, is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also, this protein is found in plasma. Over amino acids (aa) 26-1482, human PZP shares 56% aa sequence identity with mouse PZP.
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