Human Pregnancy Zone Protein/PZP Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8280
MAB8280-SP
Detection of Human Pregnancy Zone Protein/PZP by Western Blot.
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Human Pregnancy Zone Protein/PZP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pregnancy Zone Protein/PZP in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG Clone # 909813
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human Pregnancy Zone Protein/PZP
Thr26-Val1482
Accession # P20742
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB82801).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pregnancy Zone Protein/PZP DuoSet ELISA Kit (Catalog # DY8280-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Pregnancy Zone Protein/PZP by Western Blot. View Larger

Detection of Human Pregnancy Zone Protein/PZP by Western Blot. Western blot shows lysate of human pregnancy sera. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pregnancy Zone Protein/PZP at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human Pregnancy Zone Protein/PZP ELISA Standard Curve. Recombinant Human Pregnancy Zone Protein/PZP protein was serially diluted 2-fold and captured by Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB82801) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pregnancy Zone Protein/PZP Monoclonal Antibody (Catalog # MAB8280) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pregnancy Zone Protein/PZP

Pregnancy Zone Protein (PZP), also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6, is a 360 kDa secreted homodimeric glycoprotein, PZP monomer contains a region of decisive functional importance i.e. the bait region. This region serves as a substrate to a variety of proteinases, and cleavage in this domain causes a conformational change of the molecule. Human PZP is a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins.  Highest expression of human PZP is reported in liver, medium expression in ovary, heart and stomach. Also, this protein is found in plasma.  Over amino acids (aa) 26-1482, human PZP shares 56% aa sequence identity with mouse PZP.

Entrez Gene IDs
5858 (Human)
Alternate Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6; CPAMD6; Pregnancy Zone Protein; pregnancy-zone protein; PZP

Product Datasheets

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Reconstitution Buffers

Secondary Antibodies

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