Human/Primate IL-15 Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human IL-15 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, -1 beta, -2, -3, -4, -5, -6, -7, -8, -9, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 34505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-15
    Asn49-Ser162
    Accession # P40933
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human/Primate IL-15 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate IL‑15 Antibody (Catalog # MAB647)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human/Primate IL‑15 Biotinylated Antibody (Catalog # BAM247)
  • ELISA Standard
     
    Recombinant Human IL-15 Protein (Catalog # 247-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 3 ng/mL Recombinant Human IL‑15.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑15 Monoclonal Antibody (Catalog # MAB647). The ND50 is typically 4-20 ng/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
  • References:
    1. Grabstein, K. et al. (1994) Science 264:965.
    2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
    3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    5. Giri, J.G. et al. (1995) EMBO 14:3654.
    6. Giri, J. et al. (1994) EMBO J. 13:2822.
    7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
    8. Dubois, S. et al. (2002) Immunity 17:537.
    9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
    10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
    14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016;6(0):32320.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. IL-15 controls T cell functions through its influence on CD30 and OX40 antigens in Celiac Disease.
    Authors: Periolo N, Guillen L, Arruvito M, Alegre N, Niveloni S, Hwang J, Bai J, Chernavsky A
    Cytokine, 2014;67(1):44-51.
    Species: Human
    Sample Type:
  3. Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
    Authors: Saghafian-Hedengren S, Sohlberg E, Theorell J, Carvalho-Queiroz C, Nagy N, Persson J, Nilsson C, Bryceson Y, Sverremark-Ekstrom E
    J Virol, 2013;87(24):13446-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  5. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  6. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  7. Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
    Authors: Rhoton-Vlasak A, Hardt N
    Fertil. Steril., 2005;83(3):659-65.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
    Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
    Exp. Dermatol., 2004;13(12):764-72.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  9. NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
    Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
    Blood, 2004;104(10):3267-75.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004;28(10):1023-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
    Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK
    Immunology, 2000;99(3):411-7.
    Species: Human
    Sample Type: Amniotic Fluid
    Application: ELISA Development
Expand to show all 11 Citations
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Average Rating: 5 (Based on 1 review)

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ELISA Human/Primate IL-15 Antibody MAB647
ELISA: Human/Primate IL-15 Antibody [MAB647]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis antibody was used as the solid phase molecule in an ELISA for IL15. The BAM247 antibody was used as the liquid phase molecule.

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