Human Proteinase 3/Myeloblastin/PRTN3 Antibody

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Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Western Blot.
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Product Details
Citations (1)
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Human Proteinase 3/Myeloblastin/PRTN3 Antibody Summary

Species Reactivity
Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Granzyme B is observed.
Monoclonal Mouse IgG2A Clone # 684042
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3
Accession # P24158
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Proteinase 3/Myeloblastin/PRTN3 antibody by Western Blot. View Larger

Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Western Blot. Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human Proteinase 3/Myeloblastin/PRTN3 Mono-clonal Antibody (Catalog # MAB6134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proteinase 3/Myeloblastin/PRTN3

Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Entrez Gene IDs
5657 (Human); 19152 (Mouse); 314615 (Rat)
Alternate Names
ACPA; AGP7; AGP7PR3; azurophil granule protein 7; C-ANCA; EC 3.4.21; EC; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP4; NP-4; P29C-ANCA antigen; PR-3CANCA; Proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen

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Citation for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SERPINB1-mediated checkpoint of inflammatory caspase activation
    Authors: YJ Choi, S Kim, Y Choi, TB Nielsen, J Yan, A Lu, J Ruan, HR Lee, H Wu, B Spellberg, JU Jung
    Nat. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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