Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Protocadherin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Protocadherin‑1
Thr58-Asn162
Accession # Q08174

Specificity

Detects human Protocadherin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Protocadherin-8 and rhProtocadherin-10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Protocadherin‑1 Antibody

Detection of Human Protocadherin-1 antibody by Western Blot.

Detection of Human Protocadherin-1 by Western Blot.

Western blot shows lysates of human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Protocadherin-1 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Protocadherin-1 in BG01V Human Stem Cells.

Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protocadherin‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: Human hippocampus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin-1

Protocadherin-1 (PCDH-1; also PC42) is a 150 - 170 kDa delta 1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca++-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58 - 852) plus a 364 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58 - 844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There are multiple splice variants. Alternative start sites exist at Met23 and Met235, there is a deletion of aa 209 - 220, and a 27 aa substitution for aa 1034 - 1237. Over aa 58 - 162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.

Alternate Names

PC42, PCDH1, PCDH42, Protocadherin1

Entrez Gene IDs

5097 (Human); 75599 (Mouse); 307481 (Rat)

Gene Symbol

PCDH1

UniProt

Q08174

Additional Protocadherin-1 Products

Product Documents for Human Protocadherin‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protocadherin‑1 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

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Protocols

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