Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1052528
View all PTGDR Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within amino acids 50-150 of the Prostaglandin D2 receptor protein sequences
Accession # Q13258

Specificity

Detects Prostaglandin D2 receptor immunogen in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PTGDR Antibody

Detection of PTGDR in Human Colon.

PTGDR was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human PTGDR Monoclonal Antibody (Catalog # MAB11249) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PTGDR Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Colon.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTGDR

PTGDR is a G protein-coupled receptor that has been shown to function as a prostanoid DP receptor. PTGDR is expressed in retinal choroid, ciliary epithelium, longitudinal and circular ciliary muscles, iris, small intestine and platelet membranes. The activity of this receptor is mainly mediated by G-S proteins that stimulate adenylate cyclase resulting in an elevation of intracellular cAMP and Ca2+. Knockout studies in mice suggest that the ligand of PTGDR, prostaglandin D2 (PGD2), functions as a mast cell-derived mediator to trigger asthmatic responses. Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.

Long Name

Prostaglandin D2 Receptor

Alternate Names

AS1, ASRT1, DP, DP1, PTGDR1

Entrez Gene IDs

5729 (Human); 19214 (Mouse); 498475 (Rat)

Gene Symbol

PTGDR

UniProt

Q13258 (Human)

Additional PTGDR Products

Product Documents for Human PTGDR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTGDR Antibody

For research use only

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Protocols

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