Human/Rat OV-6 Antibody

(11 citations)   
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat OV-6 antigen.
  • Source
    Monoclonal Mouse IgG1 Clone # OV-6
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Carcinogen-treated rat nodular hepatocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry

    Parent, R. et al. (2004) Gastroenterology 126(4):1147.
    He, Z.P. et al. (2003) Cell Prolif. 37(2):177.

  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
OV‑6 in BG01V Human Embryonic Stem Cells. OV‑6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV‑6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OV-6
OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.
  • References:
    1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
    2. Parent, R. et al. (2004) Gastroenterology 126:1147.
    3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
    4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
    5. He, Z.P. et al. (2003) Differentation 71:281.
  • Long Name:
    Oval Cell Marker
  • Alternate Names:
    OV6; OV-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Hepatitis B virus X protein promotes the stem-like properties of OV6(+) cancer cells in hepatocellular carcinoma
    Authors: C Wang, MD Wang, P Cheng, H Huang, W Dong, WW Zhang, PP Li, C Lin, ZY Pan, MC Wu, WP Zhou
    Cell Death Dis, 2017;8(1):e2560.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Hepatic Progenitor Cells Contribute to the Progression of 2-Acetylaminofluorene/Carbon Tetrachloride-Induced Cirrhosis via the Non-Canonical Wnt Pathway.
    Authors: Chen J, Zhang X, Xu Y, Li X, Ren S, Zhou Y, Duan Y, Zern M, Zhang H, Chen G, Liu C, Mu Y, Liu P
    PLoS ONE, 2015;10(6):e0130310.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. GSK-3beta suppresses the proliferation of rat hepatic oval cells through modulating Wnt/beta-catenin signaling pathway.
    Authors: Ji X, Xie Y, Zhong J, Xu Q, Zeng Q, Wang Y, Zhang Q, Shan Y
    Acta Pharmacol Sin, 2015;36(3):334-42.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury.
    Authors: Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M
    PLoS ONE, 2015;10(8):e0134327.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  5. Stable insulin-secreting ducts formed by reprogramming of cells in the liver using a three-gene cocktail and a PPAR agonist.
    Authors: Banga, A, Greder, L V, Dutton, J R, Slack, J M W
    Gene Ther, 2014;21(1):19-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. The CD133+CD44+ precancerous subpopulation of oval cells is a therapeutic target for hepatocellular carcinoma.
    Authors: Zheng, Yun-Wen, Tsuchida, Tomonori, Shimao, Taiki, Li, Bin, Takebe, Takanori, Zhang, Ran-Ran, Sakurai, Yu, Ueno, Yasuharu, Sekine, Keisuke, Ishibashi, Naoto, Imajima, Makiko, Tanaka, Takuji, Taniguchi, Hideki
    Stem Cells Dev, 2014;23(18):2237-49.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Effects of matrine on oval cellmediated liver regeneration and expression of RBPJkappa and HES1.
    Authors: Yang Z, Wang L, Hou Y, Wang X
    Mol Med Rep, 2013;7(5):1533-8.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Expression and localization of regenerating gene I in a rat liver regeneration model.
    Authors: Wang J, Koyota S, Zhou X, Ueno Y, Ma L, Kawagoe M, Koizumi Y, Okamoto H, Sugiyama T
    Biochem. Biophys. Res. Commun., 2009;380(3):472-7.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Wnt/beta-catenin signaling contributes to activation of normal and tumorigenic liver progenitor cells.
    Authors: Yang W, Yan HX, Chen L, Liu Q, He YQ, Yu LX, Zhang SH, Huang DD, Tang L, Kong XN, Chen C, Liu SQ, Wu MC, Wang HY
    Cancer Res., 2008;68(11):4287-95.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
  10. Two pathologic types of hepatocellular carcinoma with lymph node metastasis with distinct prognosis on the basis of CK19 expression in tumor.
    Authors: Zhuang PY, Zhang JB, Zhu XD, Zhang W, Wu WZ, Tan YS, Hou J, Tang ZY, Qin LX, Sun HC
    Cancer, 2008;112(12):2740-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration.
    Authors: Dezso K, Jelnes P, László V, Baghy K, Bödör C, Paku S, Tygstrup N, Bisgaard HC, Nagy P
    Am. J. Pathol., 2007;171(5):1529-37.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 11 Citations
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