OV‑6 in BG01V Human Embryonic Stem Cells. OV‑6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV‑6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.
Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
Parent, R. et al. (2004) Gastroenterology 126:1147.
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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.