Detects human and rat PTPπ/PTPRU in Western blots and recombinant human PTPRU in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PTPRK, rhPTPRM, or rhPTPRT is observed.
Monoclonal Mouse IgG1 Clone # 764209
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human PTPπ/PTPRU Glu19-Gln740 Accession # Q92729
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat PTPπ/PTPRU by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPπ/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTPπ/PTPRU at approximately 180-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTP pi/PTPRU
Protein Tyrosine Phosphatase, receptor type U (PTPRU), also called PCP-2 or PTPp, is an approximately 180 kDa type I transmembrane glycoprotein that is a type IIb receptor-like protein tyrosine phosphatase (PTPR). The 731 amino acid (aa) extracellular domain contains MAM, Ig-like C-type and Fibronectin III domains that often mediate cell adhesion, but unlike other family members, PTPRU does not appear to mediate homophilic cell aggregation. It does, however, antagonize Wnt signaling in adherens junctions by inhibiting beta -catenin-mediated gene transcription. Human PTPRU shares 95% aa sequence identity with mouse and rat PTPRU within the extracellular domain, and up to 60% aa identity with other human family members. Isoforms of 1436, 1440 and 1433 aa are altered within the cytoplasmic domain as compared to the 1446 aa full-length form. Several tissues contain detectable amounts of PTPRU mRNA.
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