PTP pi/PTPRU Antibody (764209)

R&D Systems | Catalog # MAB7475

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 764209
View all PTP pi/PTPRU Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PTPπ/PTPRU
Glu19-Gln740
Accession # Q92729

Specificity

Detects human and rat PTPπ/PTPRU in Western blots and recombinant human PTPRU in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PTPRK, rhPTPRM, or rhPTPRT is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PTP pi/PTPRU Antibody (764209)

Detection of Human and Rat PTPp/PTPRU antibody by Western Blot.

Detection of Human and Rat PTPπ/PTPRU by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPp/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTPp/PTPRU at approximately 180-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PTP pi/PTPRU by Western Blot

Detection of Human PTP pi/PTPRU by Western Blot

An immunoblot analysis was performed to determine the expression levels of EPAS1, PTPRU and beta -catenin in response to miR-184 or miR-574-5p in SCLC cell linesA. miR-184 overexpression inhibited EPAS1 and beta -catenin in H446 and H2227 cells, whereas miR-184 inhibition had the opposite effects. B. miR-574-5p overexpression inhibited PTPRU and enhanced the tyrosine phosphorylation of beta -catenin in H446 and H2227 cells, whereas miR-574-5p inhibition had the opposite effects. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26587830), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for PTP pi/PTPRU Antibody (764209)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human brain (cerebellum) tissue and rat brain tissue

Reviewed Applications

Read 2 reviews rated 4 using MAB7475 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTP pi/PTPRU

Protein Tyrosine Phosphatase, receptor type U (PTPRU), also called PCP-2 or PTPp, is an approximately 180 kDa type I transmembrane glycoprotein that is a type IIb receptor-like protein tyrosine phosphatase (PTPR). The 731 amino acid (aa) extracellular domain contains MAM, Ig-like C-type and Fibronectin III domains that often mediate cell adhesion, but unlike other family members, PTPRU does not appear to mediate homophilic cell aggregation. It does, however, antagonize Wnt signaling in adherens junctions by inhibiting beta -catenin-mediated gene transcription. Human PTPRU shares 95% aa sequence identity with mouse and rat PTPRU within the extracellular domain, and up to 60% aa identity with other human family members. Isoforms of 1436, 1440 and 1433 aa are altered within the cytoplasmic domain as compared to the 1446 aa full-length form. Several tissues contain detectable amounts of PTPRU mRNA.

Long Name

Receptor-type Protein Tyrosine Phosphatase pi

Alternate Names

FMI, GLEPP1, PCP-2, pi R-PTP-Psi, PTP-J, PTP-PI, PTPRO, PTPRU, R-PTP-U

Entrez Gene IDs

10076 (Human); 19273 (Mouse); 116680 (Rat)

Gene Symbol

PTPRU

UniProt

Q92729 (Human)

Additional PTP pi/PTPRU Products

Product Documents for PTP pi/PTPRU Antibody (764209)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PTP pi/PTPRU Antibody (764209)

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Citations for PTP pi/PTPRU Antibody (764209)

Customer Reviews for PTP pi/PTPRU Antibody (764209) (2)

4 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human/Rat PTPπ/PTPRU Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/17/2021
    PTP pi/PTPRU Antibody (764209) MAB7475
  • Human/Rat PTPπ/PTPRU Antibody
    Name: L Taverne
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 11/04/2019
    Used a 1:250 dilution of anti-PTPRU (MAB7475) on human colon tissue section (FFPE).
    PTP pi/PTPRU Antibody (764209) MAB7475

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