Human/Rat Yes Antibody Summary
Accession # P07947.3
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Rat Yes by Western Blot. Western blot shows lysates of L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Yes in A431 Human Cell Line. Yes was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Rat Yes Antibody
There are currently no reviews for this product. Be the first to review Human/Rat Yes Antibody and earn rewards!
Have you used Human/Rat Yes Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image