RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T-cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RELT/TNFRSF19L
Ser26-Ala160 (Arg127Gly, Arg129Gly)
Accession # Q969Z4
Ser26-Ala160 (Arg127Gly, Arg129Gly)
Accession # Q969Z4
Specificity
Detects human RELT/TNFRSF19L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhTAJ, rhCD30, rhDR3, rhDR6, rhTNF RI, rhTNF RII, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, and recombinant mouse OX40 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RELT/TNFRSF19L Antibody
Detection of Human RELT/TNFRSF19L by Western Blot.
Western blot shows lysates of human bone marrow tissue and human lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RELT/TNFRSF19L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RELT/TNFRSF19L at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of RELT/TNFRSF19L by Western Blot
RELT activates cleavage of Caspase-3/7 but not PARP in MDA-MB-231 (231) cells. (A) Measurement of Caspase 3/7 activity using luciferase assay. The 231 cells were transfected with the indicated plasmids, and the amount of Caspase 3/7 cleavage was measured 48 h after transfection using a luciferase assay designed to detect Caspase 3/7 cleavage, as described in Materials and Methods. A Student’s T-test was used to determine significance: * indicates p < 0.05 between RELT and vector. (B) Measurement of Caspase 3/7 activity by flow cytometry. The 231 cells were transfected with the indicated plasmids, and Caspase 3 activity was measured via flow cytometry 48 h after transfection, as described in Materials and Methods. (C) Testing whether PARP is cleaved by RELT, RELL1, and RELL2 in MDA-MB-231 cells. The 231 cells were transfected with the indicated constructs, and Western blotting was performed 48 h after transfection, as described in Materials and Methods. A primary rabbit antibody (Ig) directed against cleaved PARP and an anti-rabbit IR 800 conjugated Ig were utilized to visualize cleaved PARP as green bands. An anti-HA antibody was used to verify the expression of recombinant RELT, RELL1, and RELL2 in 231 cells. Representatives of at least 3 experiments are shown for all results in Figure 5. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39767574), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human RELT/TNFRSF19L Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human bone marrow tissue and human lymph node tissue
Sample: Human bone marrow tissue and human lymph node tissue
Reviewed Applications
Read 1 review rated 5 using AF1385 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RELT/TNFRSF19L
References
- http://www.gene.ucl.ac.uk/nomenclature/genefamily/tnftop.html.
- Sica, G. et al. (2001) Blood 97:2702.
Long Name
Receptor Expressed in Lymphoid Tissues
Alternate Names
TNFRSF19L
Gene Symbol
RELT
UniProt
Additional RELT/TNFRSF19L Products
Product Documents for Human RELT/TNFRSF19L Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human RELT/TNFRSF19L Antibody
For research use only
Citations for Human RELT/TNFRSF19L Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
