Human RELT/TNFRSF19L Antibody

Catalog # Availability Size / Price Qty
AF1385
AF1385-SP
Human RELT/TNFRSF19L Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human RELT/TNFRSF19L Antibody Summary

Species Reactivity
Human
Specificity
Detects human RELT/TNFRSF19L in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhTAJ, rhCD30, rhDR3, rhDR6, rhTNF RI, rhTNF RII, rhEDAR, rhFas, rhGITR, rhHVEM, rhNGF R, rhOPG, and recombinant mouse OX40 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RELT/TNFRSF19L
Ser26-Ala160 (Arg127Gly, Arg129Gly)
Accession # Q969Z4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human RELT/TNFRSF19L antibody by Western Blot. View Larger

Detection of Human RELT/TNFRSF19L by Western Blot. Western blot shows lysates of human bone marrow tissue and human lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RELT/TNFRSF19L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1385) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RELT/TNFRSF19L at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T-cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.

References
Long Name
Receptor Expressed in Lymphoid Tissues
Entrez Gene IDs
84957 (Human); 320100 (Mouse)
Alternate Names
Receptor expressed in lymphoid tissues; RELT tumor necrosis factor receptor; RELT; TNFRSF19L; TNFRSF19LFLJ14993; tumor necrosis factor receptor superfamily member 19L; tumor necrosis factor receptor superfamily, member 19-like

Product Datasheets

Citations for Human RELT/TNFRSF19L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. RELT stains prominently in B-cell lymphomas and binds the hematopoietic transcription factor MDFIC
    Authors: JK Cusick, Y Alhomsy, S Wong, G Talbott, VN Uversky, C Hart, N Hejazi, AT Jacobs, Y Shi
    Biochemistry and Biophysics Reports, 2020;24(0):100868.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. RELT induces cellular death in HEK 293 epithelial cells.
    Authors: Cusick JK, Mustian A, Goldberg K, Reyland ME
    Cell. Immunol., 2010;261(1):1-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Secondary Antibodies

Reviews for Human RELT/TNFRSF19L Antibody

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Human RELT/TNFRSF19L Antibody
By Anonymous on 09/27/2017
Application: WB Sample Tested: Recombinant protein expressed in HEK293 cells Species: Human