Human Renalase Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Renalase in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Renalase
    Ala2-Ile342
    Accession # NP_001026879
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Renalase by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Renalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Renalase at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Renalase

Renalase is a novel, 35‑37 kDa FAD-dependent amine oxidase that is secreted primarily by glomerular and renal tubule epithelium. It presumably degrades catecholamines and lowers blood pressure by decreasing heart rate and cardiac contractility. In addition to renal cells, Renalase is produced by skeletal and cardiac muscle fibers. The human Renalase precursor is 342 amino acids (aa) in length. It contains an FAD binding domain (aa 4‑35) that overlaps a signal sequence (aa 1‑17), followed by an amine oxidase segment (aa 75‑339). Renalase circulates as both a monomer (from kidney) and homodimer (from heart). There is one potential splice variant that shows a 22 aa substitution for aa 294‑342. Over aa 2‑342, human Renalase shows 72% aa identity to mouse Renalase.

  • Entrez Gene IDs:
    55328 (Human); 67795 (Mouse); 361751 (Rat)
  • Alternate Names:
    C10orf59; C10orf59chromosome 10 open reading frame 59; EC 1.4; FLJ11218; MAO-C; Monoamine oxidase-C; Renalase; renalase, FAD-dependent amine oxidase; RNLS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Identification of a receptor for extracellular renalase.
    Authors: Wang, Ling, Velazquez, Heino, Chang, John, Safirstein, Robert, Desir, Gary V
    PLoS ONE, 2015;10(4):e0122932.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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