Human Resistin Antibody

Catalog # Availability Size / Price Qty
AF1359
AF1359-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Human Resistin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Resistin in direct ELISAs and Western blots. In these formats, this antibody shows less than 2% cross-reactivity with rmResistin, rmRELM alpha and rmRELM beta.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Resistin
Ser17-Pro108
Accession # Q9HD89
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Resistin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Human Resistin is an 11-12 kDa member of a small family of inflammation-related cysteine-rich polypeptides. It is named for its antagonistic activity towards insulin (resistance to insulin) and contains five intrachain disulfide bonds that generate a mixture of alpha -helices and beta -sheets. Secreted resistin forms disulfide-linked homodimers that likely oligomerize. Resistin may also form nondisulfide-linked heterodimers with RELM beta. One alternate splice form exists that shows an in-frame 26 amino acid (aa) deletion between amino acids 40 - 65. The significance is unclear. Mature human Resistin is 50% aa identical to human RELM beta and 56% aa identical to mouse Resistin.

Entrez Gene IDs
56729 (Human); 57264 (Mouse)
Alternate Names
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1

Product Datasheets

Citations for Human Resistin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members.
    Authors: Fan C, Johns B, Su Q, Kolosova I, Johns R
    Histochem Cell Biol, 2012;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  3. Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling.
    Authors: Tubbs KA, Kiernan UA, Niederkofler EE
    Anal. Chem., 2006;78(10):3271-6.
    Species: Human
    Sample Types: Plasma
    Applications: Mass Spectometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Resistin Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Resistin Antibody
By Anonymous on 12/01/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human Resistin Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

MAB13591 was used as the capture antibody in a sandwich ELISA along with AF1359 as the detection. We also tested BAF1359 as the detection since in comes biotinylated. The standard for the ELISA was 1359-RN. The assay worked excellently! It was sensitive and specific and detected all human samples we tested at a 1:4 dilution.