Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, ELISA Development, MALDI-TOF

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Resistin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Resistin
Ser17-Pro108
Accession # Q9HD89

Specificity

Detects human Resistin in direct ELISAs and Western blots. In these formats, this antibody shows less than 2% cross-reactivity with rmResistin, rmRELM alpha and rmRELM beta.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Resistin Antibody

Detection of Human Resistin by Block/Neutralize

Detection of Human Resistin by Block/Neutralize

Systemic and local HBP and resistin responses in septic patients.HBP and resistin levels in plasma collected from patients on the day of inclusion were measured with ELISA, for details see Experimental procedures. (A,B) HBP and resistin levels in plasma from patients with severe sepsis/septic shock (n = 88) or non-infected critically ill patients (n = 31). (C) Plasma HBP and resistin levels in severe sepsis/septic shock patients with confirmed Gram-positive (G+) or Gram-negative (G−) infections (n = 48). (D) Systemic HBP and resistin in STSS patients (n = 8). The correlation was determined by Pearson’s correlation test, indicated by p and r-values. (E) HBP, resistin, macrophages and neutrophils were analysed by immunohistochemical staining of cryosections of snap-frozen tissue biopsies (n = 9) from patients with severe soft tissue infections caused by GAS. A control staining where the primary antibody was omitted was also performed (control). A representative tissue biopsy is shown. The scale bar indicates 50 μm. (F) The stainings were evaluated by microscope and acquired computerized image analysis (ACIA), for details see Experimental procedures. Significant correlation was determined by Spearman test, indicated by p- and r-values. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/srep21288), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Resistin by Block/Neutralize

Detection of Human Resistin by Block/Neutralize

Subcellular localization of HBP and resistin in neutrophils.Neutrophils from healthy donors were isolated and stimulated with LPS (50 ng/ml), fMLP (5 μg/ml) or streptococcal M1-protein (1 μg/ml) for 2 h. The neutrophils were immunoflourescently stained for HBP (white), resistin (green) and MPO (red), the cell nuclei were visualized using DAPI (blue). Using scanning laser confocal microscopy, Z-stacks of the cells were acquired and vesicles positive for HBP, resistin or MPO identified using the Imaris image analysis software (processed 3D-Image). The degree of colocalization between the above-mentioned markers in the processed images was then quantified using Imaris analysis software. (A) Shows images of cells from a high responder. Bars indicate 5 μm. (B–D) Percentage of co-localization between factors as indicated. (E) Differences in co-localization in neutrophils unstimulated or stimulated with either M1-protein or LPS. Statistical significant differences were determined using the Mann Whitney test, **P < 0.01. Data represent results obtained from multiple field analyses of two experiments using different donors. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/srep21288), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Resistin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Resistin

Reviewed Applications

Read 2 reviews rated 4.5 using AF1359 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Resistin

Human Resistin is an 11-12 kDa member of a small family of inflammation-related cysteine-rich polypeptides. It is named for its antagonistic activity towards insulin (resistance to insulin) and contains five intrachain disulfide bonds that generate a mixture of alpha -helices and beta -sheets. Secreted resistin forms disulfide-linked homodimers that likely oligomerize. Resistin may also form nondisulfide-linked heterodimers with RELM beta. One alternate splice form exists that shows an in-frame 26 amino acid (aa) deletion between amino acids 40 - 65. The significance is unclear. Mature human Resistin is 50% aa identical to human RELM beta and 56% aa identical to mouse Resistin.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Gene Symbol

RETN

UniProt

Q9HD89

Additional Resistin Products

Product Documents for Human Resistin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Resistin Antibody

For research use only

Related Research Areas

Glucose Homeostasis
Obesity

Citations for Human Resistin Antibody

Customer Reviews for Human Resistin Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

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  • Human Resistin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/01/2017
  • Human Resistin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/17/2017
    MAB13591 was used as the capture antibody in a sandwich ELISA along with AF1359 as the detection. We also tested BAF1359 as the detection since in comes biotinylated. The standard for the ELISA was 1359-RN. The assay worked excellently! It was sensitive and specific and detected all human samples we tested at a 1:4 dilution.
    Human Resistin Antibody AF1359

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