Human ROR1 Antibody Summary
Ala24-Glu403
Accession # Q01973
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR1 by Western Blot. Western blot shows lysate of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human ROR1 by Simple WesternTM. Simple Western lane view shows lysates of NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROR1
ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.
Product Datasheets
Citations for Human ROR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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The Anti-ROR1 Monoclonal Antibody Zilovertamab Inhibits the Proliferation of Ovarian and Endometrial Cancer Cells
Authors: D Liu, GF Kaufmann, JB Breitmeyer, KA Dickson, DJ Marsh, CE Ford
Pharmaceutics, 2022-04-11;14(4):.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
ROR1 is upregulated in endometrial cancer and represents a novel therapeutic target
Authors: D Liu, K Gunther, LA Enriquez, B Daniels, TA O'Mara, K Tang, AB Spurdle, CE Ford
Sci Rep, 2020-08-17;10(1):13906.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway
Authors: Y Liu, H Deng, L Liang, G Zhang, J Xia, K Ding, N Tang, K Wang
Genes & diseases, 2020-07-25;8(2):232-240.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1
Authors: SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat
EBioMedicine, 2019-05-10;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
ROR1-CAVIN3 interaction required for caveolae-dependent endocytosis and pro-survival signaling in lung adenocarcinoma
Authors: T Yamaguchi, M Hayashi, L Ida, M Yamamoto, C Lu, T Kajino, J Cheng, M Nakatochi, H Isomura, M Yamazaki, M Suzuki, T Fujimoto, T Takahashi
Oncogene, 2019-03-20;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
A receptor tyrosine kinase ROR1 inhibitor (KAN0439834) induced significant apoptosis of pancreatic cells which was enhanced by erlotinib and ibrutinib
Authors: AH Daneshmane, M Hojjat-Far, A Ghaderi, A Moshfegh, L Hansson, J Schultz, J Vågberg, S Byström, E Olsson, T Olin, A Österborg, H Mellstedt
PLoS ONE, 2018-06-01;13(6):e0198038.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL
Authors: M Chow, L Gao, JD MacManiman, VT Bicocca, BH Chang, JJ Alumkal, JW Tyner
Oncogene, 2018-05-30;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
ROR1 and ROR2 play distinct and opposing roles in endometrial cancer
Authors: CE Henry, E Llamosas, B Daniels, A Coopes, K Tang, CE Ford
Gynecol. Oncol., 2018-02-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interaction between ROR1 and MuSK activation complex in myogenic cells
Authors: H Karvonen, K Summala, W Niininen, HR Barker, D Ungureanu
FEBS Lett., 2018-01-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Silencing ROR1 and ROR2 inhibits invasion and adhesion in an organotypic model of ovarian cancer metastasis
Authors: C Henry, N Hacker, C Ford
Oncotarget, 2017-11-20;8(68):112727-112738.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1
Authors: T Yamaguchi, C Lu, L Ida, K Yanagisawa, J Usukura, J Cheng, N Hotta, Y Shimada, H Isomura, M Suzuki, T Fujimoto, T Takahashi
Nat Commun, 2016-01-04;7(0):10060.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion.
Authors: Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C
Oncotarget, 2015-11-24;6(37):40310-26.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ror1 is a pseudokinase that is crucial for met-driven tumorigenesis.
Authors: Gentile A, Lazzari L, Benvenuti S, Trusolino L, Comoglio PM
Cancer Res., 2011-04-12;71(8):3132-41.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor.
Authors: Hudecek M, Schmitt TM, Baskar S
Blood, 2010-08-11;116(22):4532-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
Clin. Cancer Res., 2008-01-15;14(2):396-404.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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