Human ROR1 Antibody

Catalog # Availability Size / Price Qty
AF2000
AF2000-SP
Detection  of  ROR1 by Western Blot.
3 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews

Human ROR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROR1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ROR2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR1
Ala24-Glu403
Accession # Q01973
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
25 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of  ROR1 by Western Blot. View Larger

Detection of ROR1 by Western Blot. Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection  of ROR1 in MDA‑MB‑231 Human Cell Line by Flow  Cytometry. View Larger

Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Simple Western Detection  of Human ROR1 by Simple WesternTM. View Larger

Detection of Human ROR1 by Simple WesternTM. Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR1 at approximately 143 kDa (as indicated) using 25 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Long Name
Receptor Tyrosine Kinase-like Orphan Receptor 1
Entrez Gene IDs
4919 (Human); 26563 (Mouse)
Alternate Names
EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; ROR1; tyrosine-protein kinase transmembrane receptor ROR1

Product Datasheets

Citations for Human ROR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. A receptor tyrosine kinase ROR1 inhibitor (KAN0439834) induced significant apoptosis of pancreatic cells which was enhanced by erlotinib and ibrutinib
    Authors: AH Daneshmane, M Hojjat-Far, A Ghaderi, A Moshfegh, L Hansson, J Schultz, J Vågberg, S Byström, E Olsson, T Olin, A Österborg, H Mellstedt
    PLoS ONE, 2018;13(6):e0198038.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Maintenance and pharmacologic targeting of ROR1 protein levels via UHRF1 in t(1;19) pre-B-ALL
    Authors: M Chow, L Gao, JD MacManiman, VT Bicocca, BH Chang, JJ Alumkal, JW Tyner
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  3. Silencing ROR1 and ROR2 inhibits invasion and adhesion in an organotypic model of ovarian cancer metastasis
    Authors: C Henry, N Hacker, C Ford
    Oncotarget, 2017;8(68):112727-112738.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1
    Authors: T Yamaguchi, C Lu, L Ida, K Yanagisawa, J Usukura, J Cheng, N Hotta, Y Shimada, H Isomura, M Suzuki, T Fujimoto, T Takahashi
    Nat Commun, 2016;7(0):10060.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  5. Ror1 is a pseudokinase that is crucial for met-driven tumorigenesis.
    Authors: Gentile A, Lazzari L, Benvenuti S, Trusolino L, Comoglio PM
    Cancer Res., 2011;71(8):3132-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  6. The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor.
    Authors: Hudecek M, Schmitt TM, Baskar S
    Blood, 2010;116(22):4532-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  7. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
    Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
    Clin. Cancer Res., 2008;14(2):396-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion.
    Authors: Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C
    Oncotarget, 0;6(37):40310-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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