Detection of ROR1 by Western Blot. Western blot shows lysate of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ROR1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Goat Anti-Human ROR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2000, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ROR1 belongs to the ROR family of receptor tyrosine kinases, which are
characterized by: the extracellular immunoglobulin domain,
cysteine-rich domain that resembles the Wnt-binding sites of Frizzled
proteins, kringle domain and intracellular kinase domain. Human ROR1
shares 97% and 58% amino acid sequence identity with mouse ROR1 and
human ROR2, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.