Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 577513
View all S100A7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A7
Met1-Gln101
Accession # P31151

Specificity

Detects human S100A7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A8, A9, A10, A11, A14, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human S100A7 Antibody

S100A7 antibody in Human Skin by Immunohistochemistry (IHC-P).

S100A7 in Human Skin.

S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human S100A7 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin (hair follicle)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A7

S100A7 (also Psoriasin) is an 11-13 kDa member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-48 and 50-85), three zinc-binding His residues, one high-affinity Ca-binding site (aa 63-74) and an overlapping antibacterial sequence (aa 35-80). There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93% aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54% aa identical to S100A7.

Long Name

S100 Calcium Binding Protein A7

Alternate Names

PSOR1

Entrez Gene IDs

6278 (Human); 192344 (Mouse)

Gene Symbol

S100A7

UniProt

P31151

Additional S100A7 Products

Product Documents for Human S100A7 Antibody

Certificate of Analysis

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Product Specific Notices for Human S100A7 Antibody

For research use only

Citations for Human S100A7 Antibody

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Protocols

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