Human S100A7 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human S100A7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A8, A9, A10, A11, A14, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 577513
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human S100A7
    Met1-Gln101
    Accession # P31151
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
S100A7 in Human Skin. S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at
4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A7

S100A7 (also Psoriasin) is an 11-13 kDa member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-48 and 50-85), three zinc-binding His residues, one high-affinity Ca-binding site (aa 63-74) and an overlapping antibacterial sequence (aa 35-80). There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93% aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54% aa identical to S100A7.

  • Long Name:
    S100 Calcium Binding Protein A7
  • Entrez Gene IDs:
    6278 (Human); 192344 (Mouse)
  • Alternate Names:
    HID 5; HID5; HID-5; protein S100-A7; PSOR1; psoriasin 1; Psoriasin; S100 calcium binding protein A7 (psoriasin 1); S100 calcium binding protein A7; S100 calcium-binding protein A7 (psoriasin 1); S100 calcium-binding protein A7; S100A7; S100A7c
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