Detection of Human SCL/Tal1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein),
is a 40 kDa basic helix-loop-helix transcription factor. It regulates
hematopoiesis, angiogenesis and erythrocyte maturation. Activation of
SCL by chromosomal translocation may be a cause of some T-cell acute
lymphoblastic leukemia. Two alternative splice variants lacking the
first 25 or 175 amino acid residues also exist. Human and mouse SCL
share 93% amino acid sequence identity.
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