Human SCL/Tal1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human SCL in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SCL
    Arg199-Gly266
    Accession # P17542
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SCL/Tal1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCL/Tal1
SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein), is a 40 kDa basic helix-loop-helix transcription factor. It regulates hematopoiesis, angiogenesis and erythrocyte maturation. Activation of SCL by chromosomal translocation may be a cause of some T-cell acute lymphoblastic leukemia. Two alternative splice variants lacking the first 25 or 175 amino acid residues also exist. Human and mouse SCL share 93% amino acid sequence identity.
  • Long Name:
    Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1
  • Entrez Gene IDs:
    6886 (Human)
  • Alternate Names:
    BHLHA17; bHLHa17tal-1; Class A basic helix-loop-helix protein 17; SCL; SCLT-cell acute lymphocytic leukemia protein 1; Stem Cell Protein; Tal1; TAL-1; T-cell acute lymphocytic leukemia 1; T-cell leukemia/lymphoma protein 5; TCL5; TCL5tal-1 product
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1.
    Authors: Ishiguro K, Rice AM, Rice KP, Sartorelli AC
    Leuk. Res., 2009;33(9):1249-54.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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