Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SCL/Tal1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SCL
Arg199-Gly266
Accession # P17542

Specificity

Detects human SCL in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SCL/Tal1 Antibody

Detection of Human SCL/Tal1 antibody by Western Blot.

Detection of Human SCL/Tal1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human SCL/Tal1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCL/Tal1

SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein), is a 40 kDa basic helix-loop-helix transcription factor. It regulates hematopoiesis, angiogenesis and erythrocyte maturation. Activation of SCL by chromosomal translocation may be a cause of some T-cell acute lymphoblastic leukemia. Two alternative splice variants lacking the first 25 or 175 amino acid residues also exist. Human and mouse SCL share 93% amino acid sequence identity.

Long Name

Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1

Alternate Names

Stem Cell Protein, Tal1, TCL5

Entrez Gene IDs

6886 (Human)

Gene Symbol

TAL1

UniProt

P17542

Additional SCL/Tal1 Products

Product Documents for Human SCL/Tal1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SCL/Tal1 Antibody

For research use only

Citations for Human SCL/Tal1 Antibody

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Protocols

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