Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 254506
View all Semaphorin 6C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6C
Ala25-Val601
Accession # NP_112175

Specificity

Detects human Semaphorin 6C in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 6A, 6B, 6D, 7A, recombinant mouse Semaphorin 3C, 3E, 3F, or 6C is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Semaphorin 6C Antibody (254506)

Detection of Semaphorin 6C by Immunohistochemistry

Detection of Semaphorin 6C by Immunohistochemistry

Confocal microscopy of immunolabeled GBM control and GPC1-silenced cells for a lipid raft marker or correlated HSPGs.U-251 MG (1), C- (2), as controls cells, and C12 (3), C15, (4) and C23 (5) GPC1-depleted cells were immunolabeled for GPC1, in green, and different other antigens, in red, such as (A) GFAP, (B) FLOT1, (C) SDC4, and (D) GPC3. The cells’ nuclei were stained with DAPI, shown in blue. The images were obtained with a Leica TCS SP8 CARS confocal microscope. The scale bar refers to 100 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32180897), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Semaphorin 6C Antibody (254506)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Semaphorin 6C

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6C

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 ‑ 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that exhibit neuropilin-independent binding to specific plexin A receptors (1 ‑ 3). Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Human Sema6C cDNA encodes 930 aa, including a 24 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 306 aa cytoplasmic portion. Alternate exon splicing creates a 922 aa short form (Sema6C.3) that is lacking aa 184 - 223 within the Sema domain, but contains 32 unique aa inserted after aa 586; postnatally, this form predominates in muscle (2, 3). A 962 aa form contains only the insert. The 930 aa “long form” predominates in brain, especially in areas of increased plasticity (4). Human Sema6C ECD shares 92%, 93%, 94%, 94%, 95% and 87% aa identity with corresponding mouse, rat, porcine, bovine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is down‑regulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

References

  1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
  3. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  4. Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
  5. Yoshida, Y. et al. (2006) Neuron 52:775.
  6. Svensson, A. et al. (2008) J. Mol. Hist. 39:5.

Alternate Names

Sema6C, Semaphorin Y

Entrez Gene IDs

10500 (Human); 20360 (Mouse)

Gene Symbol

SEMA6C

UniProt

NP_112175

Additional Semaphorin 6C Products

Product Documents for Human Semaphorin 6C Antibody (254506)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Semaphorin 6C Antibody (254506)

For research use only

Related Research Areas

Proteoglycans

Citations for Human Semaphorin 6C Antibody (254506)

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