Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin B5/Maspin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin B5/Maspin
Met1-Pro375
Accession # P36952

Specificity

Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F2, rhSerpin I1, rhSerpin I2, rhPEDF, and recombinant mouse Serpin D1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Serpin B5/Maspin Antibody

Detection of Human Serpin B5/Maspin antibody by Western Blot.

Detection of Human Serpin B5/Maspin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Serpin B5/Maspin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Serpin B5/Maspin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin B5/Maspin Antibody

Application
Recommended Usage

Knockout Validated

Serpin B5/Maspin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Serpin B5/Maspin knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin B5/Maspin

Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.

Alternate Names

Maspin, PI5

Entrez Gene IDs

5268 (Human)

Gene Symbol

SERPINB5

UniProt

P36952

Additional Serpin B5/Maspin Products

Product Documents for Human Serpin B5/Maspin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin B5/Maspin Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Serpin B5/Maspin Antibody

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