Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. The amino acid sequence of human Serpin B5 is 99%, 92%, 89%, 88% and 64% identical to that of chimpanzee, dog, mouse, rat and chicken.
Human Serpin B5/Maspin Antibody
R&D Systems | Catalog # AF2218
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Knockout Validated, Western Blot
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Serpin B5/Maspin
Met1-Pro375
Accession # P36952
Met1-Pro375
Accession # P36952
Specificity
Detects human Serpin B5/Maspin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F2, rhSerpin I1, rhSerpin I2, rhPEDF, and recombinant mouse Serpin D1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Serpin B5/Maspin Antibody
Detection of Human Serpin B5/Maspin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Serpin B5/Maspin Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Serpin B5/Maspin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin B5/Maspin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2218) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin B5/Maspin at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human Serpin B5/Maspin Antibody
Application
Recommended Usage
Knockout Validated
Serpin B5/Maspin
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Serpin B5/Maspin knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑3 human prostate cancer cell line, and RT-4 human bladder carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin B5/Maspin
Additional Serpin B5/Maspin Products
Product Documents for Human Serpin B5/Maspin Antibody
Product Specific Notices for Human Serpin B5/Maspin Antibody
For research use only
Related Research Areas
Citations for Human Serpin B5/Maspin Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars