Human Serpin G1/C1 Inhibitor Antibody

Catalog # Availability Size / Price Qty
AF2488
AF2488-SP
Human Serpin G1/C1 Inhibitor Antibody in Western Blot
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Serpin G1/C1 Inhibitor Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin G1/C1 Inhibitor in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F1, rhSerpin F2, rhSerpin I1, rhSerpin I2, recombinant mouse (rm) Serpin D1 and rmSerpin E2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human plasma-derived Serpin G1/C1 Inhibitor
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Human Serpin G1/C1 Inhibitor (Catalog # 2488‑PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of   Serpin G1/C1 Inhibitor by Western Blot. View Larger

Detection of Serpin G1/C1 Inhibitor by Western Blot. Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin G1/C1 Inhibitor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin G1/C1 Inhibitor at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human Serpin G1/C1 Inhibitor by Simple  WesternTM. View Larger

Detection of Human Serpin G1/C1 Inhibitor by Simple WesternTM. Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin G1/C1 Inhibitor at approximately 162 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin G1/C1 Inhibitor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2488) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin G1/C1 Inhibitor

As a member of the serpin superfamily of serine protease inhibitors, Serpin G1/C1 inhibitor is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). Serpin G1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).

References
  1. Davis, A.E. III et al. (1993) Methods Enzymol. 223:97.
  2. Davis, A.E. III (2004) Drug News Perspect. 17:439.
Entrez Gene IDs
710 (Human)
Alternate Names
C1 Inhibitor; C1IN; C1INH; C1NH; HAE1; HAE2; Serpin G1; SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1

Product Datasheets

Citation for Human Serpin G1/C1 Inhibitor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII.
    Authors: Zakharova N, Artemenko E, Podoplelova N, Sveshnikova A, Demina I, Ataullakhanov F, Panteleev M
    PLoS ONE, 2015;10(2):e0116665.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Serpin G1/C1 Inhibitor Antibody

There are currently no reviews for this product. Be the first to review Human Serpin G1/C1 Inhibitor Antibody and earn rewards!

Have you used Human Serpin G1/C1 Inhibitor Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review