Human Serum Amyloid A1 Antibody

Catalog # Availability Size / Price Qty
MAB30191
MAB30191-SP
Human Serum Amyloid A1 Antibody in Immunohistochemistry
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Product Details
Citations (1)
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Human Serum Amyloid A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serum Amyloid A1 in direct ELISAs.
Source
Monoclonal Mouse IgG3 Clone # 902738
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Serum Amyloid A1
Accession # P0DJI8
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Serum Amyloid A1 in Human Liver. View Larger

Serum Amyloid A1 in Human Liver. Serum Amyloid A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Amyloid A1 Monoclonal Antibody (Catalog # MAB30191) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1

Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines.  It is secreted as a 12 kDa, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex (SwissProt #P02735).  The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent.  The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids.  Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus.  Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively.   

Entrez Gene IDs
6288 (Human); 20208 (Mouse)
Alternate Names
MGC111216; PIG4; SAA; SAA1; SAA2; serum amyloid A protein; Serum Amyloid A1; TP53I4; tumor protein p53 inducible protein 4

Product Datasheets

Citation for Human Serum Amyloid A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Genomic variants link to hepatitis C racial disparities
    Authors: MM Yeh, S Boukhar, B Roberts, N Dasgupta, SS Daoud
    Oncotarget, 2017;8(35):59455-59475.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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