Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 902738
View all Serum Amyloid A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serum Amyloid A1
Accession # P0DJI8

Specificity

Detects human Serum Amyloid A1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human Serum Amyloid A1 Antibody

Serum Amyloid A1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Serum Amyloid A1 in Human Liver.

Serum Amyloid A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Amyloid A1 Monoclonal Antibody (Catalog # MAB30191) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Serum Amyloid A1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 4 using MAB30191 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serum Amyloid A1

Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines.  It is secreted as a 12 kDa, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex (SwissProt #P02735).  The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent.  The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids.  Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus.  Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively.   

Alternate Names

PIG4, SAA1

Entrez Gene IDs

6288 (Human); 20208 (Mouse)

Gene Symbol

SAA1

UniProt

P0DJI8

Additional Serum Amyloid A1 Products

Product Documents for Human Serum Amyloid A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serum Amyloid A1 Antibody

For research use only

Citations for Human Serum Amyloid A1 Antibody

Customer Reviews for Human Serum Amyloid A1 Antibody (1)

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  • Human Serum Amyloid A1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 12/24/2021
    Human Serum Amyloid A1 Antibody MAB30191

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Protocols

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