Human SF20 Antibody

Catalog # Availability Size / Price Qty
AF1147
AF1147-SP
Detection of Human SF20 by Western Blot.
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Product Details
Citations (1)
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Human SF20 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SF20 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SF20
Val32-Leu173
Accession # Q969H8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SF20 by Western Blot. View Larger

Detection of Human SF20 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20 Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SF20 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF20

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted)  bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.              

Long Name
Stromal Cell-derived Growth Factor SF20
Entrez Gene IDs
56005 (Human); 28106 (Mouse)
Alternate Names
C19orf10; chromosome 19 open reading frame 10; EUROIMAGE1875335; IL-25; IL25IL27w; IL27; IL-27; interleukin 25; interleukin 27 working designation; Interleukin-25; Ly6elg; MYDGF; R33729_1; SF20; SF20hypothetical protein LOC56005; Stromal cell-derived growth factor SF20; stromal cell-derived growth factor

Product Datasheets

Citation for Human SF20 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Myeloid-derived growth factor is a resident endoplasmic reticulum protein
    Authors: V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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