Human SF20/MYDGF Antibody Summary
Val32-Leu173
Accession # Q969H8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SF20/MYDGF by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human SF20/MYDGF by Simple WesternTM. Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of SF20/MYDGF by Western Blot rhMYDGF promotes HCAEC proliferation. HCAECs were stimulated with rhMYDGF (100 ng/mL unless otherwise indicated) in the absence or presence of PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs. B, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs stimulated for 12 h. C‐D, Western blot analysis was performed to examine the protein levels of cyclin D1. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of SF20/MYDGF by Western Blot rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of SF20/MYDGF by Western Blot rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of SF20/MYDGF by Western Blot rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SF20/MYDGF
Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.
Product Datasheets
Citation for Human SF20/MYDGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Myeloid-derived growth factor is a resident endoplasmic reticulum protein
Authors: V Bortnov, DS Annis, FJ Fogerty, KT Barretto, KB Turton, DF Mosher
J. Biol. Chem., 2018-06-28;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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