Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SF20/MYDGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SF20/MYDGF
Val32-Leu173
Accession # Q969H8

Specificity

Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SF20/MYDGF Antibody

Detection of Human SF20/MYDGF antibody by Western Blot.

Detection of Human SF20/MYDGF by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human SF20/MYDGF by Simple WesternTM.

Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of SF20/MYDGF by Western Blot

Detection of SF20/MYDGF by Western Blot

rhMYDGF promotes HCAEC proliferation. HCAECs were stimulated with rhMYDGF (100 ng/mL unless otherwise indicated) in the absence or presence of PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs. B, Representative immunoblots showing cyclin D1 and GAPDH expression in HCAECs stimulated for 12 h. C‐D, Western blot analysis was performed to examine the protein levels of cyclin D1. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SF20/MYDGF by Western Blot

Detection of SF20/MYDGF by Western Blot

rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SF20/MYDGF by Western Blot

Detection of SF20/MYDGF by Western Blot

rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SF20/MYDGF by Western Blot

Detection of SF20/MYDGF by Western Blot

rhMYDGF promotes HCAEC proliferation and is unstimulated or stimulated with rhMYDGF (100 ng/mL unless otherwise indicated), PD98059 (PD, 20 µmol/L) or Static (1 nmol/L), as indicated. A, Representative immunoblots showing phosphor (p)‐MAPK1/3 (Y204/T202), MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs. B, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of MAPK1/3 inhibitor (PD98059). C, Representative immunoblots showing p‐MAPK1/3, MAPK1/3, p‐STAT3 (S727) and p‐STAT3 (Y705) expression in HCAECs in the presence of STAT3 inhibitor (Static). D‐F, Western blot analysis was performed to examine the protein levels of p‐MAPK1/3 and p‐STAT3 by ImageJ. *P < .05, **P < .01 and ***P < .001 vs baseline (two independent‐sample t test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31758636), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SF20/MYDGF Antibody

Application
Recommended Usage

Simple Western

25 µg/mL
Sample: DU145 human prostate carcinoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SF20/MYDGF

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted)  bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.              

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human); 28106 (Mouse)

Gene Symbol

MYDGF

UniProt

Q969H8

Additional SF20/MYDGF Products

Product Documents for Human SF20/MYDGF Antibody

Certificate of Analysis

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Product Specific Notices for Human SF20/MYDGF Antibody

For research use only

Citations for Human SF20/MYDGF Antibody

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