Human SIRP beta 1/CD172b Antibody Summary
Gly26-Ala369 (Arg53His and Ala363Pro)
Accession # O00241.4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SIRP-beta in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human SIRP-beta Monoclonal Antibody (Catalog # MAB20961, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
SIRP beta 1/CD172b in U937 human histiocytic lymphoma cell line. SIRP beta 1/CD172b was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human SIRP beta 1/CD172b Monoclonal Antibody (Catalog # MAB20961) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP beta 1/CD172b
SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Unlike SIRP alpha that has cytoplasmic ITIM domains, SIRP beta 1 possesses positively charged residues that allow association with ITAM motif containing adaptor molecules. SIRP beta 1 is expressed on cells of monocyte, macrophage or dendritic lineages.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human SIRP beta 1/CD172b Antibody
There are currently no reviews for this product. Be the first to review Human SIRP beta 1/CD172b Antibody and earn rewards!
Have you used Human SIRP beta 1/CD172b Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image