Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 308908
View all SIRP beta 1/CD172b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SIRP beta 1/CD172b
Gly26-Ala369 (Arg53His and Ala363Pro)
Accession # O00241.4

Specificity

Detects human SIRP beta 1/CD172b in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SIRP beta 1/CD172b Antibody

Detection of SIRP-beta antibody in Human Peripheral Blood Granulocytes antibody by Flow Cytometry.

Detection of SIRP-beta in Human Peripheral Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human SIRP-beta Monoclonal Antibody (Catalog # MAB20961, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
SIRP beta 1/CD172b antibody in U937 human histiocytic lymphoma cell line by Immunocytochemistry (ICC).

SIRP beta 1/CD172b in U937 human histiocytic lymphoma cell line.

SIRP beta 1/CD172b was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human SIRP beta 1/CD172b Monoclonal Antibody (Catalog # MAB20961) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human SIRP beta 1/CD172b Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human Peripheral Blood Granulocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 1/CD172b

SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Unlike SIRP alpha that has cytoplasmic ITIM domains, SIRP beta 1 possesses positively charged residues that allow association with ITAM motif containing adaptor molecules. SIRP beta 1 is expressed on cells of monocyte, macrophage or dendritic lineages.

Long Name

Signal-regulatory Protein beta 1

Alternate Names

CD172b, SIRPB1

Entrez Gene IDs

10326 (Human); 102139733 (Cynomolgus Monkey)

Gene Symbol

SIRPB1

UniProt

O00241.4

Additional SIRP beta 1/CD172b Products

Product Documents for Human SIRP beta 1/CD172b Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SIRP beta 1/CD172b Antibody

For research use only

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Protocols

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FAQs

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