Human SLC22A1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SLC22A1 by Western Blot.
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Product Details
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Human SLC22A1 Antibody Summary

Species Reactivity
Detects human SLC22A1 in Western blots.
Monoclonal Mouse IgG2A Clone # 609222
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human SLC22A1
Accession # NP_003048
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SLC22A1 antibody by Western Blot. View Larger

Detection of Human SLC22A1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLC22A1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry View Larger

Detection SLC22A1 in HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human SLC22A1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A control antibody (IC003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC22A1

SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80 kDa 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively.

Long Name
Solute carrier family 22 member 1
Entrez Gene IDs
6580 (Human); 24904 (Rat)
Alternate Names
hOCT1; OCT1; oct1_cds; OCT1solute carrier family 22 member 1; Organic cation transporter 1; SLC22A1; solute carrier family 22 (organic cation transporter), member 1

Product Datasheets

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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